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用于研究健康和患腐蹄病绵羊趾间皮肤的新型炎症细胞浸润评分系统。

Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin.

作者信息

Agbaje Michael, Rutland Catrin S, Maboni Grazieli, Blanchard Adam, Bexon Melissa, Stewart Ceri, Jones Michael A, Totemeyer Sabine

机构信息

School of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom.

出版信息

PeerJ. 2018 Jul 2;6:e5097. doi: 10.7717/peerj.5097. eCollection 2018.

DOI:10.7717/peerj.5097
PMID:30002960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6033080/
Abstract

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulent in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent load and was significant ( < 0.0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent load.

摘要

绵羊腐蹄病是一种绵羊足部的退行性疾病，会导致蹄角质与下层皮肤分离并引起跛行。本研究定量检查了绵羊趾间皮肤的组织学特征，以及它们与促炎细胞因子（IL-1β）和腐蹄病中病原菌的关系。从55只健康绵羊和30只患腐蹄病绵羊的足部，在屠宰后采集平行活检样本（一个用于组织学固定），并使用苏木精和伊红以及过碘酸希夫试剂进行病变分析和组织病理学分析。两种情况下的组织学病变相似，而炎症评分反映了IL-1β的表达水平。炎症评分增加与高病原菌载量相对应，与炎症评分为1和2的腐蹄病足部相比，炎症评分为3的腐蹄病足部差异显著（P<0.0001）。此外，与健康组织相比，腐蹄病样本中真细菌载量的定位超出了毛囊深度。本研究中新型的炎性细胞浸润评分系统可用于对绵羊足部的炎症反应进行分级，并证明了炎症反应严重程度与病原菌载量增加之间的关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/1ecf874730ca/peerj-06-5097-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/e5e0644d0ae7/peerj-06-5097-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/587bcaef7c61/peerj-06-5097-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/4cb24a36e184/peerj-06-5097-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/e274710c442a/peerj-06-5097-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/67356793fbb4/peerj-06-5097-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/15cc01184876/peerj-06-5097-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/1ee9bea0256e/peerj-06-5097-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/2ec1e0e31a22/peerj-06-5097-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/da309915d8f2/peerj-06-5097-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/1ecf874730ca/peerj-06-5097-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/e5e0644d0ae7/peerj-06-5097-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/587bcaef7c61/peerj-06-5097-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/4cb24a36e184/peerj-06-5097-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/e274710c442a/peerj-06-5097-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/67356793fbb4/peerj-06-5097-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/15cc01184876/peerj-06-5097-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/1ee9bea0256e/peerj-06-5097-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/2ec1e0e31a22/peerj-06-5097-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/da309915d8f2/peerj-06-5097-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9275/6033080/1ecf874730ca/peerj-06-5097-g010.jpg

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