Tian Wenli, Wang Dan, Fan Haoran, Yang Lujuan, Ma Gang
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, China.
Front Chem. 2018 Jun 28;6:261. doi: 10.3389/fchem.2018.00261. eCollection 2018.
In this work, we explored to use chemometrics-based Fourier transform infrared (FTIR) spectroscopy to investigate the plasma biochemical changes due to acute lead poisoning (ALP) in a rat model. We first collected the FTIR spectra of the plasma samples from the rats with and without suffering from ALP. We then performed the chemometric analysis of these FTIR spectra using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). We found that the chemometrics-based FTIR spectroscopy can discriminate the rats with and without ALP. Further analysis on the PLS-DA regression coefficient revealed that the spectral changes, in particular, corresponding to the biochemical changes of proteins in the plasma may be used as potential spectral biomarkers for the diagnostics of lead poisoning. Our work demonstrates the potential of chemometrics-based FTIR spectroscopy as a promising tool for the biochemical analysis of plasma that could consequently enable an objective, convenient and non-destructive diagnostics of lead poisoning. To the best of our knowledge, this work is the first application of chemometrics-based FTIR spectroscopy in the diagnostics of lead poisoning.
在本研究中，我们探索使用基于化学计量学的傅里叶变换红外（FTIR）光谱技术，来研究大鼠急性铅中毒（ALP）模型中血浆的生化变化。我们首先采集了患和未患ALP大鼠的血浆样本的FTIR光谱。然后，我们使用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对这些FTIR光谱进行化学计量学分析。我们发现，基于化学计量学的FTIR光谱能够区分患和未患ALP的大鼠。对PLS-DA回归系数的进一步分析表明，光谱变化，特别是与血浆中蛋白质生化变化相对应的部分，可用作铅中毒诊断的潜在光谱生物标志物。我们的研究证明了基于化学计量学的FTIR光谱作为血浆生化分析的一种有前景的工具的潜力，这进而能够实现对铅中毒的客观、便捷且无损的诊断。据我们所知，本研究是基于化学计量学的FTIR光谱在铅中毒诊断中的首次应用。
