用于高效单细胞捕获、按需分隔和下游核酸分析的自数字化介电泳（SD-DEP）芯片。
A Self-Digitization Dielectrophoretic (SD-DEP) Chip for High-Efficiency Single-Cell Capture, On-Demand Compartmentalization, and Downstream Nucleic Acid Analysis.
机构信息
Department of Chemistry, University of Washington, Seattle, Washington, 98195, USA.
Department of Chemistry, Iowa State University, Ames, Iowa, 50010, USA.
出版信息
Angew Chem Int Ed Engl. 2018 Aug 27;57(35):11378-11383. doi: 10.1002/anie.201807314. Epub 2018 Jul 27.
Abstract
The design and fabrication of a self-digitization dielectrophoretic (SD-DEP) chip with simple components for single-cell manipulation and downstream nucleic acid analysis is presented. The device employed the traditional DEP and insulator DEP to create the local electric field that is tailored to approximately the size of single cells, enabling highly efficient single-cell capture. The multistep procedures of cell manipulation, compartmentalization, lysis, and analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, decreasing lysate dilution, and increasing assay sensitivity. The platform developed here could be a promising and powerful tool in single-cell research for precise medicine.
摘要
本文提出了一种具有简单组件的自数字化介电泳（SD-DEP）芯片的设计和制造，用于单细胞操作和下游核酸分析。该器件采用传统的DEP 和电介质 DEP 来创建局部电场，该电场被调整到大约单细胞的大小，从而实现高效的单细胞捕获。在集成微器件中进行了多步细胞操作、分隔、裂解和分析，消耗的试剂最少，污染最小，裂解液稀释最小，检测灵敏度增加。这里开发的平台可以成为单细胞研究中用于精准医学的有前途和强大的工具。
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