Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China.
Sensors (Basel). 2024 Sep 30;24(19):6359. doi: 10.3390/s24196359.
Cellular heterogeneity plays a significant role in understanding biological processes, such as cell cycle and disease progression. Microfluidics has emerged as a versatile tool for manipulating single cells and analyzing their heterogeneity with the merits of precise fluid control, small sample consumption, easy integration, and high throughput. Specifically, integrating microfluidics with electrical techniques provides a rapid, label-free, and non-invasive way to investigate cellular heterogeneity at the single-cell level. Here, we review the recent development of microfluidic-based electrical strategies for single-cell manipulation and analysis, including dielectrophoresis- and electroporation-based single-cell manipulation, impedance- and AC electrokinetic-based methods, and electrochemical-based single-cell detection methods. Finally, the challenges and future perspectives of the microfluidic-based electrical techniques for single-cell analysis are proposed.
细胞异质性在理解生物学过程（如细胞周期和疾病进展）方面起着重要作用。微流控技术已成为一种用于操纵单细胞和分析其异质性的多功能工具，具有精确控制流体、小样本消耗、易于集成和高通量的优点。具体来说，将微流控技术与电子技术相结合，提供了一种快速、无标记和非侵入式的方法，可在单细胞水平上研究细胞异质性。在这里，我们综述了基于微流控的单细胞操作和分析的电策略的最新进展，包括基于介电泳和电穿孔的单细胞操作、基于阻抗和交流电动的方法以及基于电化学的单细胞检测方法。最后，提出了基于微流控的电技术在单细胞分析方面面临的挑战和未来展望。
