Deng Bo, Wang Kun, Huang Peng, Yang Miaomiao, Liu Demeng, Guan Yimin
School of Microelectronics, Shanghai University, Shanghai 201800, China.
Shanghai Aurefluidics Technology Co. Ltd, Shanghai 201800, China.
Biomicrofluidics. 2024 Nov 26;18(6):064102. doi: 10.1063/5.0225883. eCollection 2024 Dec.
Single-cell printing technology has arisen as a potent instrument for investigating cell biology and disease pathophysiology. Nonetheless, current single-cell printing methodologies are hindered by restricted throughput, a limited field of view, and diminished efficiency. We present an innovative single-cell printing chip that utilizes thermal inkjet technology for single-cell printing, therefore addressing these constraints. We have accomplished high-throughput, wide-field, and efficient single-cell printing by merging a high-density thermal foam-based inkjet nozzle array on a chip with high-speed cameras and computer vision technologies for optical image capture and single-cell identification training. We have shown the efficacy and adaptability of the printing chip by printing various concentrations of Chinese hamster ovary cells and human embryonic kidney 293 cells. The printing of a single 96-well plate is accomplished in 2-3 min, facilitating one-time loading and uninterrupted multi-plate paving. Our thermal bubble single-cell printing chip serves as a viable platform for high-throughput single-cell analysis applications.
单细胞打印技术已成为研究细胞生物学和疾病病理生理学的有力工具。尽管如此，当前的单细胞打印方法受到通量受限、视野有限和效率降低的阻碍。我们提出了一种创新的单细胞打印芯片，该芯片利用热喷墨技术进行单细胞打印，从而解决了这些限制。通过将基于高密度热泡沫的喷墨喷嘴阵列集成在芯片上，并结合高速相机和用于光学图像捕获及单细胞识别训练的计算机视觉技术，我们实现了高通量、宽视野和高效的单细胞打印。我们通过打印不同浓度的中国仓鼠卵巢细胞和人胚肾293细胞，展示了打印芯片的有效性和适应性。单个96孔板的打印在2 - 3分钟内完成，便于一次性加载和连续多板铺板。我们的热泡单细胞打印芯片是高通量单细胞分析应用的可行平台。
