Zhang Lin, Ma Xinxin, Dong Youhong
Department of Pharmacy, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang 441000, P.R. China.
J BUON. 2018 May-Jun;23(3):641-646.
To investigate the effect of genistein (GEN) on the apoptosis of lung adenocarcinoma cells and the expression of Bcl-2 and Bax, so as to screen effective antitumor drugs for the clinical treatment of lung adenocarcinoma.
Lung adenocarcinoma A549 cells were cultured in vitro and treated with different concentrations of GEN. The untreated cells were set as control group. MTT assay and Annexin V/PI double staining were used to analyze cell proliferation and apoptosis after GEN treatment. RT-qPCR and Western blot were used to detect the expression of Bcl-2 and Bax at mRNA and protein levels, respectively.
Cell p roliferation a ssay s howed t hat GEN c ould inhibit the proliferation of lung adenocarcinoma cell line A549 in vitro in a dose-dependent manner. Cell apoptosis assay showed that, compared with the control group, the apoptotic rate of A549 cells was significantly increased after GEN treatment. RT-qPCR and Western blot showed that the expression levels of anti-apoptotic factor Bcl-2 in A549 cells were significantly decreased at both mRNA and protein levels at 48 hrs after treatment with GEN, but the levels of pro-apoptotic Bax were significantly increased at both mRNA and protein levels.
GEN can inhibit the proliferation of A549 lung adenocarcinoma cells in vitro and induce their apoptosis. The antitumor activity of GEN is achieved by downregulating Bcl-2 and upregulating Bax. Therefore, GEN can be applied to the clinical treatment of lung adenocarcinoma.
探讨金雀异黄素（GEN）对肺腺癌细胞凋亡及Bcl-2和Bax表达的影响，为肺腺癌临床治疗筛选有效的抗肿瘤药物。
体外培养肺腺癌A549细胞，用不同浓度的GEN处理，将未处理的细胞设为对照组。采用MTT法和Annexin V/PI双染法分析GEN处理后细胞的增殖和凋亡情况。分别用RT-qPCR和Western blot检测Bcl-2和Bax在mRNA和蛋白水平的表达。
细胞增殖实验表明，GEN能在体外以剂量依赖的方式抑制肺腺癌细胞系A549的增殖。细胞凋亡实验表明，与对照组相比，GEN处理后A549细胞的凋亡率显著增加。RT-qPCR和Western blot显示，GEN处理48小时后，A549细胞中抗凋亡因子Bcl-2在mRNA和蛋白水平的表达均显著降低，但促凋亡因子Bax在mRNA和蛋白水平均显著升高。
GEN可在体外抑制A549肺腺癌细胞的增殖并诱导其凋亡。GEN的抗肿瘤活性是通过下调Bcl-2和上调Bax来实现的。因此，GEN可应用于肺腺癌的临床治疗。
