Wang Pingping, Sun Wenqing, Wang Longxia, Gao Jie, Zhang Jie, He Ping
Department of Ultrasound, Beijing Luhe Hospital Affiliated to Capital Medical University, Beijing 101149, China.
J BUON. 2018 May-Jun;23(3):769-775.
To investigate the correlations of p53 expression with transvaginal color Doppler ultrasound findings of cervical cancer after radiotherapy (RT).
A total of 78 patients with cervical cancer (stage II and III) treated in the Oncology Department of our hospital from March 2011 to September 2017 were enrolled, and another 10 normal cervical tissue specimens were taken from the Pathology Department as controls. RT was performed to the 78 enrolled patients. Morphological features of tumor tissues after RT were detected via hematoxylineosin (HE) staining, the mutant p53 protein level was detected via immunohistochemistry (IHC), and imaging signs and blood flow resistance index (RI) of cervical cancer were detected via transvaginal color Doppler ultrasound. Finally, the correlations of p53 protein with transvaginal color Doppler ultrasound findings were analyzed.
After RT, most cervical cancer tissues showed nuclear degeneration, karyolysis, cytoplasmic keratosis (vacuolization), and regeneration and fibrosis of cancer tissues. The expression of p53 was negative in normal cervix, while there were 48 p53-positive cases (61.54%) and 30 p53-negative cases (38.46%) in patients with cervical cancer (p<0.05). No echo was detected in 2 out of 78 patients, and there were 4 cases of equal echo, 36 cases of low echo and 36 cases of high echo. Results of x2 test showed that the positive rate of p53 protein was significantly correlated with cervical space-occupying lesion and mass diameter shown in transvaginal color Doppler ultrasound (p<0.05), but it had no significant correlation with pelvic lymph node metastasis (p>0.05). The p53 protein expression level was significantly correlated with color Doppler flow imaging (CDFI) grading and RI (p<0.05).
The p53 protein expression in cervical cancer after RT is significantly correlated with cervical space-occupying lesion and tumor size shown in transvaginal color Doppler ultrasound, CDFI grading and RI. p53 level and transvaginal color Doppler ultrasound can provide certain valuable clinical information for the treatment and monitoring of cervical cancer.
探讨放疗（RT）后宫颈癌组织中p53表达与经阴道彩色多普勒超声检查结果的相关性。
选取2011年3月至2017年9月在我院肿瘤科治疗的78例宫颈癌患者（Ⅱ期和Ⅲ期），另取10例正常宫颈组织标本作为对照。对78例入选患者进行放疗。放疗后肿瘤组织的形态学特征采用苏木精-伊红（HE）染色检测，突变型p53蛋白水平采用免疫组织化学（IHC）检测，宫颈癌的影像学征象和血流阻力指数（RI）采用经阴道彩色多普勒超声检测。最后，分析p53蛋白与经阴道彩色多普勒超声检查结果的相关性。
放疗后，多数宫颈癌组织表现为细胞核变性、核溶解、细胞质角化（空泡化）以及癌组织的再生和纤维化。正常宫颈组织中p53表达为阴性，而宫颈癌患者中p53阳性病例48例（61.54%），p53阴性病例30例（38.46%）（p<0.05）。78例患者中2例未检测到回声，4例等回声，36例低回声，36例高回声。χ²检验结果显示，p53蛋白阳性率与经阴道彩色多普勒超声显示的宫颈占位性病变及肿块直径显著相关（p<0.05），但与盆腔淋巴结转移无显著相关性（p>0.05）。p53蛋白表达水平与彩色多普勒血流成像（CDFI）分级及RI显著相关（p<0.05）。
放疗后宫颈癌组织中p53蛋白表达与经阴道彩色多普勒超声显示的宫颈占位性病变、肿瘤大小以及CDFI分级和RI显著相关。p53水平和经阴道彩色多普勒超声可为宫颈癌的治疗和监测提供一定的有价值的临床信息。
