Zhan Zhigao, Xiao Yuzhou, Liu Zhuorong, Zhang Wenchao, Wang Jinchang, Wang Hongxiu, Guan Limei, Yang Gang, Huang Zhao, Jin Liang
Bing Du Xue Bao. 2016 Sep;32(5):619-26.
In order to develop a novel effective biological insecticide for controlling oleander hawk moth, a new pathogen was isolated from naturally diseased Daphnis nerii. Based on scanning electron microscopy, full-length amplification of cDNAs (FLAC), and phylogenetic analysis of genome segments 2and 10,the virus was identified as a new type of cypovirus (Da phnis nerii cypovirus [DnCPV]). Electrophoresis analysis showed that DnCPV had a genome comprising 10double-stranded RNA (dsRNA) segments, ranging from 892 to 4160bp.Using FLAC, the cDNAs from the 10 dsRNA segments of the new CPV were cloned and genome segments 2and 10 were sequenced. Sequencing results showed that segment 2 encoded RNA-dependent-RNA-polymerases (RdRps) and segment 10 encoded polyhedrin. These two segments shared conserved terminal sequences of AGUCAAA and AGC at the 5’and 3’ends,respectively.These conserved terminal sequences were not consistent with any of the known CPV types.Phylogenetic analysis of the RdRp and polyhedrin indicated that this CPV was more closely related to CPV type 19 and type 5than other CPV types. Based on the unique conserved terminal sequences and the electrophoresis pattern of the new virus, we tentatively named it DnCPV Nanchang isolate: DnCPV-NC.
为开发一种新型有效的生物杀虫剂来防治夹竹桃天蛾，从自然染病的夹竹桃天蛾中分离出一种新病原体。基于扫描电子显微镜、cDNA全长扩增（FLAC）以及基因组片段2和10的系统发育分析，该病毒被鉴定为一种新型质型多角体病毒（夹竹桃天蛾质型多角体病毒[DnCPV]）。电泳分析表明，DnCPV的基因组由10条双链RNA（dsRNA）片段组成，长度在892至4160bp之间。利用FLAC，克隆了新CPV的10条dsRNA片段的cDNA，并对基因组片段2和10进行了测序。测序结果显示，片段2编码RNA依赖的RNA聚合酶（RdRps），片段10编码多角体蛋白。这两个片段在5'和3'端分别具有保守的末端序列AGUCAAA和AGC。这些保守的末端序列与任何已知的CPV类型均不一致。RdRp和多角体蛋白的系统发育分析表明，该CPV与19型和5型CPV的关系比其他CPV类型更为密切。基于新病毒独特的保守末端序列和电泳图谱，我们暂定将其命名为DnCPV南昌分离株：DnCPV-NC。
