Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330029, China.
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
J Invertebr Pathol. 2019 May;163:43-47. doi: 10.1016/j.jip.2019.03.002. Epub 2019 Mar 8.
The oleander hawk moth, Daphnis nerii, is a serious pest of plants belonging to the family Apocynaceae. Thus far, pathogen infection has not been reported in D. nerii. In this study, a new cytoplasmic polyhedrosis virus (cypovirus; CPV) was isolated from naturally diseased D. nerii larvae and named DnCPV-23. Virions were observed in ultrathin sections of DnCPV polyhedral bodies. Electrophoretic analysis revealed that the DnCPV genome consisted of 10 segments of double-stranded RNA (dsRNA). cDNA copies of these dsRNA segments were amplified using the method of full-length amplification of cDNAs (FLAC), cloned, and sequenced. Sequencing results showed that all segments contained one open reading frame (ORF); They shared the conserved terminal sequences AGUCAAA and AGC at 5' and 3' ends respectively, except segment 4, which is different from previously reported 22 cypoviruses. Phylogenetic analysis based on amino acid sequences of polyhedrin (encoded by segment 10) indicated that this CPV was closely related to CPV type 19. Altogether, DnCPV-23 is a new type of cypovirus.
夹竹桃天蛾，Daphnis nerii，是夹竹桃科植物的一种严重害虫。迄今为止，尚未有关于 D. nerii 的病原体感染的报道。在本研究中，从自然患病的 D. nerii 幼虫中分离到一种新的细胞质多角体病毒（cypovirus；CPV），并将其命名为 DnCPV-23。在 DnCPV 多角体的超薄切片中观察到病毒粒子。电泳分析显示，DnCPV 基因组由 10 个双链 RNA（dsRNA）片段组成。使用全长 cDNA 扩增法（FLAC）扩增这些 dsRNA 片段的 cDNA 拷贝，进行克隆和测序。测序结果表明，所有片段均包含一个开放阅读框（ORF）；它们在 5'和 3'端分别具有保守的末端序列 AGUCAAA 和 AGC，除了片段 4，与先前报道的 22 种 CPV 不同。基于多角体（由第 10 个片段编码）氨基酸序列的系统发育分析表明，这种 CPV 与 CPV 类型 19 密切相关。总之，DnCPV-23 是一种新型的 CPV。
