Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Bangkok 10400, Thailand.
Department of Pre-clinical and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand; The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Infect Genet Evol. 2018 Nov;65:107-111. doi: 10.1016/j.meegid.2018.07.007. Epub 2018 Jul 9.
Blastocystis is a common intestinal pathogen of humans and a variety of animals, with various host-specific subtypes. The aim of this study was to determine the prevalence and subtype distribution of Blastocystis in humans and domestic animals, Thailand. 113 stool samples were collected from pigs, goats, and cattle in Ayutthaya Province (AP; central Thailand) and 218 stool samples were collected from pigs, dogs, cats, chickens, and humans in Kanchanaburi Province (KP; western Thailand). Blastocystis was detected by nested PCR targeting the SSU rRNA gene. Subtypes were identified by DNA sequencing, and phylogenetic analysis was conducted. The overall prevalence of Blastocystis in animals was 76.1% (86/113) and 11.88% (12/101) in AP and KP, respectively, and the prevalence in humans was 12.82% (15/117) in KP. The prevalence of Blastocystis in the AP and KP pigs were 87.88% (29/33) and 20.37% (11/54), respectively. Blastocystis ST5 was the most abundant in pigs in both areas while Blastocystis ST10 and ST12 were most frequently found in cattle and goats. In addition, low percentage of Blastocystis ST1 and Blastocystis ST14 were found in pigs and goats, respectively. In this study, Blastocystis ST3, followed by ST2 and ST1 were predominantly found in humans. In conclusion, pigs may be a natural host of Blastocystis and this ST may be the pig-adapted ST in the studied areas, in this study.
人芽囊原虫是一种常见的人类和多种动物的肠道病原体，具有各种宿主特异性亚型。本研究旨在确定芽囊原虫在泰国的人类和家畜中的流行率和亚型分布。从位于泰国中部的阿育他耶府（AP）的猪、山羊和牛中收集了 113 份粪便样本，从位于泰国西部的北碧府（KP）的猪、狗、猫、鸡和人中收集了 218 份粪便样本。通过针对 SSU rRNA 基因的巢式 PCR 检测芽囊原虫。通过 DNA 测序鉴定亚型，并进行系统发育分析。动物中芽囊原虫的总流行率分别为 AP 地区的 76.1%（86/113）和 KP 地区的 11.88%（12/101），而人类中的流行率分别为 KP 地区的 12.82%（15/117）。AP 和 KP 地区猪的芽囊原虫流行率分别为 87.88%（29/33）和 20.37%（11/54）。在这两个地区，芽囊原虫 ST5 是猪中最丰富的，而芽囊原虫 ST10 和 ST12 则在牛和山羊中最常见。此外，在猪和山羊中发现了低比例的芽囊原虫 ST1 和芽囊原虫 ST14。在本研究中，芽囊原虫 ST3，其次是 ST2 和 ST1，在人类中占主导地位。总之，猪可能是芽囊原虫的天然宿主，在本研究中，这种 ST 可能是研究地区中猪适应的 ST。
