Department of Crop Science, Chungnam National University, 99, Daehak-Ro, Yuseong-gu, Daejeon 34134, Korea.
Asia Seed Co., Ltd., 109-35, 518 Beon-gil, Gyeongchungdae-ro, Janghowon-eup, Icheon-si 17414, Korea.
Molecules. 2018 Jul 2;23(7):1613. doi: 10.3390/molecules23071613.
Pakchoi ( subsp. ) is cultivated for its nutritional value, particularly with regard to vitamins, minerals and dietary fibers. However, limited metabolic information is available on the phyto-nutritional traits of pakchoi. Our GC-TOF MS analysis showed that green pakchoi has higher contents of carbon metabolism-associated metabolites such as sugars, sugar derivatives and inositol, while purple pakchoi has higher levels of nitrogen metabolism-associated metabolites such as amino acids and amino acid derivatives. To compare the content and composition of secondary metabolites in green and purple pakchoi, we analyzed phenylpropanoid-derived compounds and anthocyanins in mature leaves using an HPLC-UV system. This analysis identified 9 phenylpropanoid-derived compounds and 12 anthocyanins in the mature leaves of green and purple pakchoi. The level of rutin was significantly higher in purple pakchoi compared with green pakchoi, consistent with the expression of phenylpropanoid biosynthetic genes in the two pakchoi cultivars. The data obtained from this comprehensive metabolic profiling would be helpful to improve our understanding of the nutritional values of pakchoi cultivars as food sources.
白菜（亚种）因其营养价值而被栽培，特别是维生素、矿物质和膳食纤维。然而，白菜的植物营养特性的代谢信息有限。我们的 GC-TOF MS 分析表明，绿色白菜含有更高含量的与碳代谢相关的代谢物，如糖、糖衍生物和肌醇，而紫色白菜含有更高水平的与氮代谢相关的代谢物，如氨基酸和氨基酸衍生物。为了比较绿色和紫色白菜中次生代谢物的含量和组成，我们使用 HPLC-UV 系统分析了成熟叶片中的苯丙烷衍生化合物和花青素。该分析在绿色和紫色白菜的成熟叶片中鉴定出 9 种苯丙烷衍生化合物和 12 种花青素。与绿色白菜相比，紫色白菜中芦丁的含量明显更高，这与两种白菜品种中苯丙烷生物合成基因的表达一致。从这项综合代谢分析中获得的数据将有助于提高我们对白菜品种作为食物来源的营养价值的理解。
