Department of Orthopedic, The 97th Hospital of the PLA, Xuzhou, China.
Eur Rev Med Pharmacol Sci. 2018 Jul;22(1 Suppl):76-82. doi: 10.26355/eurrev_201807_15367.
The objective of the present study was to investigate the correlation between polymorphisms of the back-2 gene and osteoarthritis.
We enrolled 76 patients with osteoarthritis who were admitted to our hospital between February 2014 and February 2015 for treatment as the observation group, and 46 healthy subjects as the control group. The analysis of back-2 gene polymorphisms (rs28502) was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). mRNA expression of the different genotypes was measured with reverse transcriptase-polymerase chain reaction (RT-PCR), and the protein expression of back-2 of different genotypes was measured with enzyme-linked immunosorbent assay (ELISA) and Western blotting.
At locus 173 of the back-2 gene, there were a total of three genotypes, i.e. CC, CT, and TT. The frequencies of these genotypes in healthy subjects and osteoarthritis patients were 9.5%, 82.2%, 8.3% and 47.4%, 7.5%, 45.1%, respectively. There was a significant difference (p<0.05). However, the frequency of C/T in healthy older subjects and osteoarthritis patients was 50.6%, 49.4%, 51.15%, 48.85%, respectively, and there was no significant difference (p>0.05). RT-PCR showed no significant difference in mRNA expressions of the back-2 gene between the control group and observation group (p>0.05), although ELISA indicated that the protein expression of back-2 (12.3±0.36 μg/L) in osteoarthritis patients was significantly higher than in healthy subjects (1.52±0.18 μg/L) (p<0.05). Moreover, Western blotting analysis indicated that the protein expression of back-2 in osteoarthritis patients was significantly higher than in healthy subjects.
Genetic polymorphisms of back-2 are associated with the metabolic syndrome in older people, i.e. older people with the CC or TT genotypes may be at high risk for metabolic syndrome.
本研究旨在探讨 back-2 基因多态性与骨关节炎的相关性。
我们纳入了 2014 年 2 月至 2015 年 2 月期间因治疗而在我院就诊的 76 例骨关节炎患者作为观察组，并纳入 46 名健康受试者作为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 back-2 基因多态性（rs28502）。采用逆转录-聚合酶链反应（RT-PCR）测定不同基因型的 back-2 mRNA 表达，采用酶联免疫吸附试验（ELISA）和 Western blot 测定不同基因型 back-2 的蛋白表达。
在 back-2 基因的 173 位位点，共有三种基因型，即 CC、CT 和 TT。健康受试者和骨关节炎患者的这些基因型频率分别为 9.5%、82.2%、8.3%和 47.4%、7.5%、45.1%，差异有统计学意义（p<0.05）。然而，健康老年受试者和骨关节炎患者的 C/T 频率分别为 50.6%、49.4%、51.15%、48.85%，差异无统计学意义（p>0.05）。RT-PCR 显示，对照组和观察组之间 back-2 基因的 mRNA 表达无显著差异（p>0.05），尽管 ELISA 表明骨关节炎患者的 back-2 蛋白表达（12.3±0.36 μg/L）明显高于健康受试者（1.52±0.18 μg/L）（p<0.05）。此外，Western blot 分析表明，骨关节炎患者的 back-2 蛋白表达明显高于健康受试者。
back-2 基因的遗传多态性与老年人的代谢综合征有关，即 CC 或 TT 基因型的老年人可能有患代谢综合征的高风险。
