Jiang Fan, Liu Ning, Chen Xiao Zhuang, Han Kun Yuan, Zhu Cai Zhong
Department of Geratology, Hainan General Hospital, Haikou, Hainan 570311, P.R. China.
Department of General Surgery, Hainan General Hospital, Haikou, Hainan 570311, P.R. China.
Exp Ther Med. 2017 Sep;14(3):2031-2035. doi: 10.3892/etm.2017.4714. Epub 2017 Jun 30.
The aim of the study was to examine the correlation between KCNJ11 gene polymorphism and metabolic syndrome in elderly patients. From January 2014 to January 2015, 54 elderly patients with metabolic syndrome were enrolled in this study as the observation group. During the same period, 46 healthy elderly individuals were enrolled in this study as the control group. KCNJ11 gene polymorphism (rs28502) was analyzed using polymerase chain reaction-restriction fragment length polymorphism. The expression levels of mRNA in different genotypes were detected using FQ-PCR. ELISA was used to evaluate the KCNJ11 protein expression in different genotypes. KCNJ11 gene polymorphism and metabolic syndrome was studied by measuring the blood pressure levels in patients with different genotypes. Three genotypes of gene in rs28502 were CC, CT and TT. The CC, CT and TT genotype frequencies in healthy population were 8.5, 9.2 and 82.2%, respectively, while the genotype frequencies in patients with metabolic syndrome were 42.4, 49.8 and 7.8%, respectively. There were significant differences between groups (P≤0.05). However, the genotype frequencies of C/T in healthy individuals and metabolic syndrome patients were 35.3 and 38.3%, respectively. There were no significant differences between groups (P>0.05). FQ-PCR results showed that the KCNJ11 mRNA expression levels in the control and observation groups had no significant differences (P>0.05). However, the results obtained from ELISA analysis revealed that KCNJ11 protein expression level in the observation group was significantly higher than that in the control group (P<0.05). In conclusion, KCNJ11 gene polymorphism is associated with metabolic syndrome in the elderly. Elderly patients with the CC and TT genotypes are more likely to develop metabolic syndrome.
本研究旨在探讨老年患者中KCNJ11基因多态性与代谢综合征之间的相关性。2014年1月至2015年1月，本研究纳入了54例老年代谢综合征患者作为观察组。同期，纳入46例健康老年个体作为对照组。采用聚合酶链反应-限制性片段长度多态性分析KCNJ11基因多态性（rs28502）。使用荧光定量聚合酶链反应检测不同基因型中mRNA的表达水平。采用酶联免疫吸附测定法评估不同基因型中KCNJ11蛋白的表达。通过测量不同基因型患者的血压水平研究KCNJ11基因多态性与代谢综合征的关系。rs28502基因的三种基因型为CC、CT和TT。健康人群中CC、CT和TT基因型频率分别为8.5%、9.2%和82.2%，而代谢综合征患者中的基因型频率分别为42.4%、49.8%和7.8%。组间差异有统计学意义（P≤0.05）。然而，健康个体和代谢综合征患者中C/T基因型频率分别为35.3%和38.3%。组间差异无统计学意义（P>0.05）。荧光定量聚合酶链反应结果显示，对照组和观察组中KCNJ11 mRNA表达水平差异无统计学意义（P>0.05）。然而，酶联免疫吸附测定分析结果显示，观察组中KCNJ11蛋白表达水平显著高于对照组（P<0.05）。总之，KCNJ11基因多态性与老年代谢综合征相关。CC和TT基因型的老年患者更易发生代谢综合征。
