1 Department of Hepatobiliary Surgery, The First People's Hospital of Neijiang , Neijiang, Sichuang, China .
2 Department of Anesthesiology, The First People's Hospital of Neijiang , Neijiang, Sichuang, China .
Cancer Biother Radiopharm. 2018 Nov;33(9):373-379. doi: 10.1089/cbr.2018.2500. Epub 2018 Jul 13.
To analyze the expression of micro-RNA 143 (miRNA-143) in the patients with pancreatic cancer and to explore the influence of overexpression of miRNA-143 on pancreatic cancer cells.
Twenty-five patients with pancreatic cancer that received treatment in their hospital were included in this study. Pancreatic cancer tissues of the patients were surgically removed, and cancer-adjacent tissues were taken for control. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expressions of miRNA-143 and its target gene Kirsten rat sarcoma (KRAS) in pancreatic cancer tissues and cancer-adjacent tissues. Western blot test was conducted to detect the expression of KRAS in pancreatic cancer tissues. Clinicopathologic data of patients were recorded in detail, and the analysis on the correlation of these data with the expression of miRNA-143 in pancreatic cancer tissues was conducted. Pancreatic cancer cell line (PANC-1) was transfected with plasmid for overexpression of miRNA-143, and the influences of miRNA-143 on the apoptosis, migration ability, and invasion ability of the cells were verified by flow cytometry, cell scratch experiment, and Transwell experiment.
The results of semiquantitative RT-PCR showed that there were significantly lower expression of miRNA-143 (p < 0.01) and remarkably higher expressions of KRAS gene and KRAS protein (p < 0.01) in pancreatic cancer tissues compared with cancer-adjacent tissues. The expression of miRNA-143 had no correlation with the patient's age and gender, but was closely related to the tumor size, clinical staging, and metastasis of lymph nodes. The detection with flow cytometry showed that cell apoptosis was significantly increased by overexpression of miRNA-143 in PANC-1 (p < 0.01). The result of cell scratch experiment indicated that the migration ability of PANC-1 was reduced significantly by overexpression of miRNA-143 (p < 0.01) and that of Transwell experiment manifested that the invasion ability of PANC-1 was decreased significantly by overexpression of miRNA-143 (p < 0.01).
The expression of miRNA-143 in pancreatic cancer tissues is significantly decreased. MiRNA-143 can promote cell apoptosis and regulate the process of pancreatic cancer by inhibiting the migration and invasion of pancreatic cancer cells.
分析微小 RNA 143（miRNA-143）在胰腺癌患者中的表达情况，并探讨过表达 miRNA-143 对胰腺癌细胞的影响。
选取在我院治疗的 25 例胰腺癌患者作为研究对象，手术切除患者的胰腺癌组织，同时取癌旁组织作为对照。采用半定量逆转录-聚合酶链反应（RT-PCR）检测胰腺癌组织和癌旁组织中 miRNA-143 及其靶基因 Kirsten 大鼠肉瘤（KRAS）的表达，Western blot 检测胰腺癌组织中 KRAS 的表达。详细记录患者的临床病理资料，并分析这些资料与胰腺癌组织中 miRNA-143 表达的相关性。将质粒转染入胰腺癌细胞系（PANC-1）以过表达 miRNA-143，通过流式细胞术、细胞划痕实验和 Transwell 实验验证 miRNA-143 对细胞凋亡、迁移和侵袭能力的影响。
半定量 RT-PCR 结果显示，与癌旁组织相比，胰腺癌组织中 miRNA-143 的表达显著降低（p<0.01），KRAS 基因和 KRAS 蛋白的表达显著升高（p<0.01）。miRNA-143 的表达与患者的年龄和性别无关，而与肿瘤大小、临床分期和淋巴结转移密切相关。流式细胞术检测结果显示，过表达 miRNA-143 可显著促进 PANC-1 细胞凋亡（p<0.01）。细胞划痕实验结果表明，过表达 miRNA-143 可显著降低 PANC-1 的迁移能力（p<0.01），Transwell 实验结果表明，过表达 miRNA-143 可显著降低 PANC-1 的侵袭能力（p<0.01）。
胰腺癌组织中 miRNA-143 的表达显著降低。miRNA-143 通过抑制胰腺癌细胞的迁移和侵袭，促进细胞凋亡，调节胰腺癌的发生发展过程。
