Wang ShiChao, Zhou HuanMin, Zhang RuiJian, Zhang YanRu
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, People's Republic of China.
Department of Neurosurgery, The People's Hospital of Inner Mongolia, Hohhot, Inner Mongolia, 010017, People's Republic of China.
Int J Gen Med. 2021 Nov 16;14:8281-8292. doi: 10.2147/IJGM.S336421. eCollection 2021.
Glioblastoma multiforme (GBM) is a common, malignant brain tumor in adults, with a median survival of only 15-23 months. Organisms respond to disease stress through sophisticated mechanisms at the physiological, transcriptional and metabolic levels. However, the molecular regulatory networks responsible for occurrence, progression and recurrence of glioma have yet to be elucidated.
In this study, we sought to determine the cause of gliomas by developing an RNA-seq technique that analyzes mRNA and small RNA (sRNA) with the aim of discovering potential methods for precisely blocking key signaling pathways in occurrence, progression, and recurrence. The explication of mechanisms leading to GBM formation has become a feasible and promising new therapeutic method.
GBM-associated genes were identified based on their expression during the disease stress response. Analysis of the inverse correlations between microRNAs (miRNAs) and target mRNAs revealed 43 mRNA-miRNA interactions during disease progression. BOC-SMO and BOC-RAS were found to promote the malignant progression of glioma. A total of 3088 differentially expressed genes were identified as involved in several biological processes, such as amino acid metabolism, protein transport associated with immune response, cell proliferation, and cell apoptosis. Fifteen miRNAs were also identified as being differentially expressed in GBM and control groups.
The results of this study provide an important foundation for understanding the pathogenesis of glioma and discovering new therapeutic targets.
多形性胶质母细胞瘤（GBM）是成人常见的恶性脑肿瘤，中位生存期仅为15 - 23个月。生物体通过生理、转录和代谢水平的复杂机制对疾病应激做出反应。然而，负责胶质瘤发生、进展和复发的分子调控网络尚未阐明。
在本研究中，我们试图通过开发一种RNA测序技术来确定胶质瘤的病因，该技术分析mRNA和小RNA（sRNA），旨在发现精确阻断发生、进展和复发中关键信号通路的潜在方法。阐明导致GBM形成的机制已成为一种可行且有前景的新治疗方法。
基于疾病应激反应期间的表达鉴定出GBM相关基因。对微小RNA（miRNA）与靶mRNA之间的负相关分析揭示了疾病进展过程中的43种mRNA - miRNA相互作用。发现BOC - SMO和BOC - RAS促进胶质瘤的恶性进展。共鉴定出3088个差异表达基因参与多个生物学过程，如氨基酸代谢、与免疫反应相关的蛋白质转运、细胞增殖和细胞凋亡。还鉴定出15种miRNA在GBM组和对照组中差异表达。
本研究结果为理解胶质瘤发病机制和发现新的治疗靶点提供了重要基础。
