Hornicek F J, Malinin G I, Gratzner H G, Malinin T I
Chem Biol Interact. 1985 Nov;55(3):289-302. doi: 10.1016/s0009-2797(85)80136-8.
Nitroxyldisulfonate [Fremy's salt; (KSO3)2NO.] and bisulfite (NaHSO3) have abolished periodic acid (H5IO6)-induced blastogenesis of human peripheral blood lymphocytes (HPBL), but only inhibited the blastogenic response of H5IO6-oxidized rat and mouse lymphocytes, as determined by the rates of nucleic acids synthesis, BrdUrd incorporation and by cell numbers in S + G2 + M phases of the cell cycle. The viability of the intact human, rat and mouse lymphocytes remained essentially unimpaired by 30 min pulses of 1 mM Fremy's salt or bisulfite. The marked inhibition of periodic acid-induced blastogenesis, exerted by Fremy's salt and by bisulfite, was attributed to the effect of the corresponding carbonyl addition derivatives formed in situ of the oxidized cell membranes. Consequently, it is concluded that Fremy's salt like bisulfite possibly forms addition derivatives with membrane carbonyls of viable target cells.
硝氧基二磺酸盐[弗雷米盐；(KSO3)2NO.]和亚硫酸氢盐(NaHSO3)可消除高碘酸(H5IO6)诱导的人外周血淋巴细胞(HPBL)的增殖，但根据核酸合成速率、BrdUrd掺入以及细胞周期S + G2 + M期的细胞数量测定，它们仅抑制H5IO6氧化的大鼠和小鼠淋巴细胞的增殖反应。1 mM弗雷米盐或亚硫酸氢盐脉冲处理30分钟后，完整的人、大鼠和小鼠淋巴细胞的活力基本未受损害。弗雷米盐和亚硫酸氢盐对高碘酸诱导的增殖的显著抑制作用归因于氧化细胞膜原位形成的相应羰基加成衍生物的作用。因此，可以得出结论，弗雷米盐与亚硫酸氢盐类似，可能与活靶细胞的膜羰基形成加成衍生物。
