Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, China.
Millet Research Institute, Shanxi Academy of Agricultural Sciences, Changzhi, 046011, Shanxi Province, China.
Theor Appl Genet. 2018 Oct;131(10):2145-2156. doi: 10.1007/s00122-018-3143-1. Epub 2018 Jul 13.
Key message Nine transgenes from different categories, viz. plant defense response genes and anti-apoptosis genes, played combined roles in maize to inhibit the necrotrophic pathogens Rhizoctonia solani and Bipolaris maydis. Maize sheath blight and southern corn leaf blight are major global threats to maize production. The management of these necrotrophic pathogens has encountered limited success due to the characteristics of their lifestyle. Here, we presented a transgenic pyramiding breeding strategy to achieve nine different resistance genes integrated in one transgenic maize line to combat different aspects of necrotrophic pathogens. These nine genes, selected from two different categories, plant defense response genes (Chi, Glu, Ace-AMP1, Tlp, Rs-AFP2, ZmPROPEP1 and Pti4), and anti-apoptosis genes (Iap and p35), were successfully transferred into maize and further implicated in resistance against the necrotrophic pathogens Rhizoctonia solani and Bipolaris maydis. Furthermore, the transgenic maize line 910, with high expression levels of the nine integrated genes, was selected from 49 lines. Under greenhouse and field trial conditions, line 910 showed significant resistance against maize sheath blight and southern corn leaf blight diseases. Higher-level resistance was obtained after the pyramiding of more resistance transgenes from different categories that function via different mechanisms. The present study provides a successful strategy for the management of necrotrophic pathogens.
关键信息 来自不同类别的 9 个转基因，即植物防御反应基因和抗细胞凋亡基因，在玉米中共同发挥作用，抑制坏死性病原菌立枯丝核菌和弯孢叶斑病菌。玉米叶斑病和南方玉米叶斑病是全球玉米生产的主要威胁。由于这些坏死性病原菌生活方式的特点，对它们的管理仅取得了有限的成功。在这里，我们提出了一种转基因聚合育种策略，将 9 个不同的抗性基因整合到一个转基因玉米品系中，以对抗不同方面的坏死性病原菌。这些从两个不同类别中选择的 9 个基因，植物防御反应基因（Chi、Glu、Ace-AMP1、Tlp、Rs-AFP2、ZmPROPEP1 和 Pti4）和抗细胞凋亡基因（Iap 和 p35），成功地转入玉米，并进一步对坏死性病原菌立枯丝核菌和弯孢叶斑病菌表现出抗性。此外，从 49 个品系中选择了高表达 9 个整合基因的转基因玉米品系 910。在温室和田间试验条件下，品系 910 对玉米叶斑病和南方玉米叶斑病表现出显著的抗性。通过不同机制作用的不同类别更多抗性转基因的聚合，获得了更高水平的抗性。本研究为坏死性病原菌的管理提供了一种成功的策略。
