The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2020 Jan 18;21(2):647. doi: 10.3390/ijms21020647.
Wheat ( L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the gene, encoding DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi and , the pathogenic causes of wheat sharp eyespot and common root rot. was introduced into a wheat variety Zhoumai18 via -mediated transformation. The molecular characteristics indicated that could be heritable and expressed in five transgenic wheat lines in T-T generations. Average sharp eyespot infection types of these five transgenic wheat lines in T-T generations decreased 0.69-1.54 and 0.40-0.82 compared with non-transformed Zhoumai18, respectively. Average common root rot infection types of these transgenic lines and non-transformed Zhoumai18 were 1.23-1.48 and 2.27, respectively. These results indicated that DmAMP1W-expressing transgenic wheat lines displayed enhanced-resistance to both sharp eyespot and common root rot. This study provides new broad-spectrum antifungal resources for wheat breeding.
小麦（L.）是一种重要的主食作物。眼斑病和普通根腐病是小麦的破坏性疾病。抗菌肽（AMPs）是具有广谱抗菌活性的小肽。本研究合成了编码 DmAMP1 的基因，并研究了 DmAMP1W 在体外和转基因小麦中的抗真菌作用。蛋白电泳分析和体外抑制结果表明，合成的基因正确翻译为预期的肽 DmAMP1W，纯化的肽抑制了真菌 和 的生长，这两种真菌是小麦眼斑病和普通根腐病的致病菌。 通过介导转化将 导入小麦品种周麦 18。分子特征表明， 可遗传并在 T-T 代的五个转基因小麦系中表达。与非转化周麦 18 相比，T-T 代这五个 转基因小麦系的平均眼斑病感染类型分别降低了 0.69-1.54 和 0.40-0.82。这些转基因系和非转化周麦 18 的平均普通根腐病感染类型分别为 1.23-1.48 和 2.27。这些结果表明，表达 DmAMP1W 的转基因小麦系对眼斑病和普通根腐病表现出增强的抗性。本研究为小麦育种提供了新的广谱抗真菌资源。
