CNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, UPS, LAAS, F-31400 Toulouse, France.
LCPQ-IRSAMC, Université de Toulouse, 118 Route de Narbonne, F-31062 Toulouse, France.
Biosens Bioelectron. 2019 May 1;132:90-96. doi: 10.1016/j.bios.2019.01.065. Epub 2019 Feb 19.
The present work is dedicated to the development of a lab-on-chip (LOC) device for water toxicity environmental analysis and more especially herbicide detection. The final goal is focused on the functional integration of three-electrode electrochemical microcells (ElecCell) and organic photodetectors (OPD) in order to perform simultaneously electrochemical and optical detection in the frame of algal metabolism monitoring. Considering three different algae, ie. Chlamydomonas reinhardtii, Pseudokirchneriella subcapitata and Chlorella vulgaris while dealing with photosynthesis, the multi-microsensor platform enables to measure the variations of microalgae fluorescence as well as oxygen production. It is applied to study the Diuron herbicide influences on algal metabolism, evidencing fluorescence enhancement and oxygen production inhibition for concentrations as low as few tens of nanomoles. These results are performed with unconcentrated and six time concentrated algae solutions respectively, to estimate the ability of this dual-sensor system to conduct measurements without any sample preparation. Thus, according to the obtained results, the proposed LOC device is fully adapted to the electrochemical/optical dual detection for on-site pollutant analysis, ie. without sample pre-treatment.
本工作致力于开发一种用于水质毒性环境分析的微流控芯片（LOC）设备，特别是用于除草剂检测。最终目标是将三电极电化学微电池（ElecCell）和有机光电探测器（OPD）功能集成，以便在藻类代谢监测框架内同时进行电化学和光学检测。在考虑三种不同藻类，即莱茵衣藻、假鱼腥藻和普通小球藻的光合作用时，多微传感器平台能够测量微藻荧光的变化以及氧气的产生。该平台用于研究除草剂 Diuron 对藻类代谢的影响，结果表明，低至数十纳摩尔浓度的 Diuron 就会增强荧光并抑制氧气的产生。这些结果分别使用未经浓缩和浓缩六倍的藻类溶液进行了验证，以评估该双传感器系统在无需任何样品制备的情况下进行测量的能力。因此，根据获得的结果，所提出的 LOC 设备完全适用于现场污染物分析的电化学/光学双重检测，即无需样品预处理。
