健康个体中抗胃壁细胞自身抗体的流行率估计因方法而异。
Estimate of the Prevalence of Anti-Gastric Parietal Cell Autoantibodies in Healthy Individuals Is Method Dependent.
机构信息
Dipartimento di Medicina Interna, University of Genoa, Genoa, Italy.
Laboratory of Autoimmunology, Ospedale Policlinico San Martino, Genoa, Italy.
出版信息
Am J Clin Pathol. 2018 Aug 30;150(4):285-292. doi: 10.1093/ajcp/aqy061.
OBJECTIVES
Anti-parietal cell antibodies (APCA) are a serologic marker of autoimmune gastritis. Their prevalence in healthy individuals is not well defined.
METHODS
We evaluated APCA prevalence in 515 healthy blood-donors by rat/primate tissue indirect immunofluorescence (IIF), enzyme-linked immunosorbent assay (ELISA), and immunoblot.
RESULTS
Fifty-three of 515 (10.3%) subjects were positive for APCA by at least one method: 18 only by ELISA, 10 by rodent tissue IIF, and one by primate tissue IIF; 18 were positive by ELISA and primate tissue IIF, and one by ELISA and rodent tissue IIF. Two were positive by both IIF methods, and three were triple positive. APCA positivity was confirmed by immunoblot in 100% of ELISA positive, in 95.8% of positive primate tissue IIF, and in 50% of positive rat tissue IIF.
CONCLUSIONS
A great discrepancy in APCA prevalence detected by different methods in this cohort was apparent. Thus, the results on APCA prevalence in healthy individuals are likely method-dependent.
目的
壁细胞抗体（APCA）是自身免疫性胃炎的血清学标志物。其在健康人群中的流行情况尚未明确。
方法
我们通过大鼠/灵长类动物组织间接免疫荧光（IIF）、酶联免疫吸附试验（ELISA）和免疫印迹法评估了 515 名健康献血者的 APCA 患病率。
结果
515 名受试者中，有 53 名（10.3%）至少有一种方法检测出 APCA 阳性：18 名仅 ELISA 阳性，10 名啮齿动物组织 IIF 阳性，1 名灵长类动物组织 IIF 阳性；18 名 ELISA 和灵长类动物组织 IIF 阳性，1 名 ELISA 和啮齿动物组织 IIF 阳性。有 2 名 IIF 两种方法均为阳性，3 名为三阳性。在 ELISA 阳性的患者中，100%的患者可通过免疫印迹法确认 APCA 阳性，95.8%的灵长类动物组织 IIF 阳性患者和 50%的啮齿动物组织 IIF 阳性患者可通过免疫印迹法确认 APCA 阳性。
结论
本研究中不同方法检测到的 APCA 患病率存在很大差异。因此，健康人群中 APCA 患病率的结果可能取决于检测方法。
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