de Oliveira Amanda Rodrigues, Oliveira Lucas Nojosa, Chaves Edilânia Gomes Araújo, Weber Simone Schneider, Bailão Alexandre Melo, Parente-Rocha Juliana Alves, Baeza Lilian Cristiane, de Almeida Soares Célia Maria, Borges Clayton Luiz
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil; Programa de Pós-graduação em Patologia Molecular, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
Fungal Biol. 2018 Aug;122(8):738-751. doi: 10.1016/j.funbio.2018.04.001. Epub 2018 Apr 11.
Paracoccidioides is a thermodimorphic fungus that causes Paracoccidioidomycosis (PCM) - an endemic systemic mycosis in Latin America. The genus comprises several phylogenetic species which present some genetic and serological differences. The diversity presented among isolates of the same genus has been explored in several microorganisms. There have also been attempts to clarify differences that might be related to virulence existing in isolates that cause the same disease. In this work, we analyzed the secretome of two isolates in the Paracoccidioides genus, isolates Pb01 and PbEpm83, and performed infection assays in macrophages to evaluate the influence of the secretomes of those isolates upon an in vitro model of infection. The use of a label-free proteomics approach (LC-MS) allowed us to identify 92 proteins that are secreted by those strains. Of those proteins, 35 were differentially secreted in Pb01, and 36 in PbEpm83. According to the functional annotation, most of the identified proteins are related to adhesion and virulence processes. These results provide evidence that different members of the Paracoccidioides complex can quantitatively secrete different proteins, which may influence the characteristics of virulence, as well as host-related processes.
副球孢子菌是一种引起副球孢子菌病（PCM）的双态真菌，PCM是拉丁美洲的一种地方性系统性真菌病。该属包括几个系统发育种，它们存在一些遗传和血清学差异。同一属分离株之间呈现的多样性已在多种微生物中得到研究。也有人试图阐明可能与引起相同疾病的分离株中存在的毒力相关的差异。在这项研究中，我们分析了副球孢子菌属中两个分离株Pb01和PbEpm83的分泌蛋白组，并在巨噬细胞中进行感染试验，以评估这些分离株的分泌蛋白组对体外感染模型的影响。使用无标记蛋白质组学方法（液相色谱-质谱联用）使我们能够鉴定出这些菌株分泌的92种蛋白质。在这些蛋白质中，35种在Pb01中差异分泌，36种在PbEpm83中差异分泌。根据功能注释，大多数鉴定出的蛋白质与黏附及毒力过程有关。这些结果提供了证据，表明副球孢子菌复合体的不同成员可定量分泌不同的蛋白质，这可能会影响毒力特征以及与宿主相关的过程。
