Department of Pharmaceutics and Pharmaceutical Nanotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran.
Department of Pharmaceutics, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
IET Nanobiotechnol. 2024 May 15;2024:6024411. doi: 10.1049/2024/6024411. eCollection 2024.
Global concerns due to the negative impacts of untreatable wounds, as well as the growing population of these patients, emphasize the critical need for advancements in the wound healing materials and techniques. Nanotechnology offers encouraging avenues for improving wound healing process. In this context, nanoparticles (NPs) and certain natural materials, including chitosan (CS) and aloe vera (AV), have demonstrated the potential to promote healing effects. The objective of this investigation is to assess the effect of novel fabricated nanocomposite gel containing CS, AV, and zinc oxide NPs (ZnO NPs) on the wound healing process. The ZnO NPs were synthesized and characterized by X-ray diffraction and electron microscopy. Then, CS/AV gel with different ratios was prepared and loaded with ZnO NPs. The obtained formulations were characterized based on an antimicrobial study, and the best formulations were used for the animal study to assess their wound healing effects in 21 days. The ZnO NPs were produced with an average 33 nm particle size and exhibited rod shape morphology. Prepared gels were homogenous with good spreadability, and CS/AV/ZnO NPs formulations showed higher antimicrobial effects against , . The wound healing findings showed significant wound area reduction in the CS/AV/ZnO NPs group compared to negative control at day 21. Histopathological assessment revealed the advantageous impact of this formulation across various stages of the wound healing process, including collagen deposition (CS/AV/ZnO NPs (2 : 1), 76.6 ± 3.3 compared to negative control, 46.2 ± 3.7) and epitheliogenesis (CS/AV/ZnO NPs (2 : 1), 3 ± 0.9 compared to negative control, 0.8 ± 0.8). CS/AV gel-loaded ZnO NPs showed significant effectiveness in wound healing and would be suggested as a promising formulation in the wound healing process. Further assessments are warranted to ensure the robustness of our findings.
由于无法治愈的伤口所带来的负面影响以及此类患者人数的不断增加，全球都在关注这一问题，这也强调了在伤口愈合材料和技术方面取得进展的紧迫性。纳米技术为改善伤口愈合过程提供了有希望的途径。在这种情况下，纳米颗粒（NPs）和某些天然材料，包括壳聚糖（CS）和芦荟（AV），已显示出促进愈合作用的潜力。本研究旨在评估含有 CS、AV 和氧化锌 NPs（ZnO NPs）的新型复合纳米凝胶对伤口愈合过程的影响。通过 X 射线衍射和电子显微镜对 ZnO NPs 进行了合成和表征。然后，制备了具有不同比例的 CS/AV 凝胶，并负载了 ZnO NPs。根据抗菌研究对获得的配方进行了表征，并将最佳配方用于动物研究，以评估其在 21 天内的伤口愈合效果。通过 X 射线衍射和电子显微镜对 ZnO NPs 进行了合成和表征。然后，制备了具有不同比例的 CS/AV 凝胶，并负载了 ZnO NPs。根据抗菌研究对获得的配方进行了表征，并将最佳配方用于动物研究，以评估其在 21 天内的伤口愈合效果。通过 X 射线衍射和电子显微镜对 ZnO NPs 进行了合成和表征。然后，制备了具有不同比例的 CS/AV 凝胶，并负载了 ZnO NPs。根据抗菌研究对获得的配方进行了表征，并将最佳配方用于动物研究，以评估其在 21 天内的伤口愈合效果。制备的凝胶均匀，具有良好的铺展性，CS/AV/ZnO NPs 配方对 、 显示出更高的抗菌效果。伤口愈合结果表明，与阴性对照组相比，CS/AV/ZnO NPs 组在第 21 天伤口面积明显减少。组织病理学评估显示，该配方在伤口愈合过程的各个阶段都具有优势，包括胶原沉积（CS/AV/ZnO NPs（2:1），76.6±3.3 与阴性对照相比，46.2±3.7）和上皮形成（CS/AV/ZnO NPs（2:1），3±0.9 与阴性对照相比，0.8±0.8）。负载 ZnO NPs 的 CS/AV 凝胶显示出在伤口愈合方面的显著效果，有望成为伤口愈合过程中的一种有前途的配方。需要进一步评估以确保我们研究结果的稳健性。
