Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brazil.
Departamento de Química, UFPR, Curitiba, Paraná, Brazil.
Carbohydr Polym. 2018 Oct 1;197:631-640. doi: 10.1016/j.carbpol.2018.06.041. Epub 2018 Jun 15.
Native (F2) and carboxyl-reduced (R) ulvans from Ulva fasciata were sequentially oxidized with periodate-chlorite affording the polycarboxyl ulvans C1, C2 and C3 (1.20, 1.41 and 1.81 mmol g of COOH, respectively; 19.7, 21.3 and 21.0% of NaSO, respectively) and R-C3 (1.86 mmol g of COOH; NaSO = 22.7%), respectively. APTT assay (polysaccharide fractions at 150 μg mL) showed clotting time of 45.6 s for F2 fraction. For polycarboxyl ulvans C1, C2, C3 and R-C3 the clotting times were 101.0, 122.2, 222.0 and 227.0 s, respectively. Comparison of the APTT assay results using ulvans chemically modified by carboxyl-reduction, desulfation, periodate oxidation and/or chlorite oxidation showed the anticoagulant activity of polycarboxyl ulvans is dependent of the sulfate groups present in the native polymer. In addition, the increase of the anticoagulant activity was accompanied by the increasing of the carboxyl groups and the content of this acidic substituent seems to be more important than its positioning.
天然（F2）和羧基还原（R）浒苔聚糖依次用过碘酸盐-次氯酸盐氧化，得到多羧基浒苔聚糖 C1、C2 和 C3（分别为 1.20、1.41 和 1.81mmol/g COOH；分别为 19.7、21.3 和 21.0%的 NaSO）和 R-C3（1.86mmol/g COOH；NaSO=22.7%）。APTT 测定（多糖组分在 150μg/mL 时）显示 F2 部分的凝血时间为 45.6s。对于多羧基浒苔聚糖 C1、C2、C3 和 R-C3，凝血时间分别为 101.0、122.2、222.0 和 227.0s。对通过羧基还原、脱硫酸、过碘酸盐氧化和/或次氯酸盐氧化化学修饰的浒苔聚糖进行 APTT 测定结果的比较表明，多羧基浒苔聚糖的抗凝活性取决于天然聚合物中存在的硫酸根。此外，抗凝活性的增加伴随着羧基数量的增加，并且这种酸性取代基的含量似乎比其位置更为重要。
