Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS 79070-900, Brazil.
Laboratório de Biofilmes e Diversidade Microbiana (LaBDiM), Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90040-060, Brazil; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS 90050-170, Brazil.
Food Res Int. 2018 Sep;111:661-673. doi: 10.1016/j.foodres.2018.05.033. Epub 2018 May 23.
Propolis, a resin produced by bees, is widely used in industrial products, including food, cosmetics, supplements, and pharmaceuticals. Extracts (ethanolic and hydroethanolic) and fractions, yielded by accelerated solvent extraction methodology, were obtained from different samples of Brazilian brown propolis (BBP). They were evaluated for antioxidant capacity, antibacterial, antibiofilm, and anti-Trichomonas vaginalis activities. The metabolomics profiling was determined by LC-DAD-MS and an innovative application of statistical analyses (univariate and chemometrics) was applied to correlate chemical compounds with biological activities. Eighty-six compounds were identified, including phenylpropanoic acids, flavonoids, chlorogenic acids, and prenylated phenylpropanoic acids. Propolis-fractions killed about 93% of Staphylococcus aureus in biofilm (at concentration of 125 μg/mL), showed activity against T. vaginalis with MIC at 400 μg/mL and significative antioxidant capacity (IC 2.32-3.80 μg/mL). Propolis extracts and fractions did not show antibacterial and antibiofilm activities against Pseudomonas aeruginosa. The prenylated phenylpropanoic acids positively correlated with both the antibiofilm (S. aureus) and anti-T. vaginalis activities, such as the metabolites artepillin C, drupanin, and baccharin.
蜂胶是蜜蜂产生的树脂，广泛应用于工业产品，包括食品、化妆品、保健品和药品。采用加速溶剂萃取方法从不同的巴西棕蜂胶（BBP）样品中提取（乙醇和水乙醇）和馏分，并对其进行抗氧化能力、抗菌、抗生物膜和抗阴道毛滴虫活性评估。采用 LC-DAD-MS 进行代谢组学分析，并应用统计分析（单变量和化学计量学）的创新应用将化合物与生物活性相关联。鉴定出 86 种化合物，包括苯丙酸类、类黄酮、绿原酸和prenylated 苯丙酸类。蜂胶馏分在生物膜（浓度为 125μg/mL）中杀死约 93%的金黄色葡萄球菌，对阴道毛滴虫的 MIC 为 400μg/mL，具有显著的抗氧化能力（IC 2.32-3.80μg/mL）。蜂胶提取物和馏分对铜绿假单胞菌没有抗菌和抗生物膜活性。prenylated 苯丙酸类与抗生物膜（金黄色葡萄球菌）和抗阴道毛滴虫活性呈正相关，如代谢物 artepillin C、drupanin 和 baccharin。
