Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare, Alice 5700, Eastern Cape Province, South Africa.
Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare, Alice 5700, Eastern Cape Province, South Africa.
J Integr Med. 2018 Sep;16(5):335-341. doi: 10.1016/j.joim.2018.07.003. Epub 2018 Jul 4.
This study investigated the acute and subacute toxicity of whole-plant aqueous extract of Vernonia mespilifolia Less. (AEVM) in rats for evaluating its safety profile.
AEVM for the acute (2000 and 5000 mg/kg) and subacute (200, 400 and 600 mg/kg) toxicity studies was administered orally to rats according the guidelines 425 and 407 of Organization for Economic Cooperation and Development, respectively. Food and water intake as well as body and organ weight of animals were recorded. Signs of toxicity were assessed, and hematological, biochemical and histopathological analyses were performed.
In the acute toxicity study, a single dose of the aqueous extract at 2000 or 5000 mg/kg caused no mortality in the animals, suggesting that the median lethal dose is greater than 5000 mg/kg. In the subacute toxicity study, administration of the extract for 28 d, at all doses, caused no significant changes in the body weights or organ weights of rats in the treated groups when compared with the control group. In addition, hematological and biochemical parameters also revealed no toxic effects of the extract on rats. Histological sections of the heart, liver and kidney from test animals showed no signs of degeneration.
These results showed that AEVM at dosage levels up to 600 mg/kg is nontoxic and could also offer protection on some body tissues. AEVM could, therefore, be considered safe.
本研究旨在调查 Vernonia mespilifolia Less. 全株水提物（AEVM）在大鼠中的急性和亚急性毒性，以评估其安全性。
根据经济合作与发展组织（OECD）425 和 407 指南，分别以 2000 和 5000mg/kg 的剂量经口给予 AEVM 进行急性（2000 和 5000mg/kg）和亚急性（200、400 和 600mg/kg）毒性研究。记录动物的食物和水摄入量以及体重和器官重量。评估毒性症状，并进行血液学、生化学和组织病理学分析。
在急性毒性研究中，单次给予 2000 或 5000mg/kg 的水提物未导致动物死亡，表明中值致死剂量大于 5000mg/kg。在亚急性毒性研究中，与对照组相比，所有剂量的提取物在 28 天的给药期间，对处理组大鼠的体重或器官重量均无显著影响。此外，血液学和生化学参数也未显示提取物对大鼠有任何毒性作用。受试动物的心脏、肝脏和肾脏组织切片均未显示退化迹象。
这些结果表明，AEVM 在高达 600mg/kg 的剂量下无毒性，并且还可以对一些身体组织提供保护。因此，可以认为 AEVM 是安全的。
