基于 ESIPT 酞酰亚胺衍生物的钯物种检测的溶酶体靶向荧光探针。
A lysosome targetable fluorescent probe for palladium species detection base on an ESIPT phthalimide derivative.
机构信息
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative innovation center for efficient and health production of fisheries in Hunan province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative innovation center for efficient and health production of fisheries in Hunan province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Dec 5;205:66-71. doi: 10.1016/j.saa.2018.06.104. Epub 2018 Jul 7.
A novel lysosome-targetable phthalimide fluorescent probe was designed for detecting palladium based on ESIPT for signal transduction. The fluorescent probe conjugating with allylcarbamate displayed weak fluorescent due to the ESIPT process hinder by allylcarbamate. But with the addition of palladium, the ESIPT emission was recovery though the palladium-catalyzed deallylation reaction and the fluorescence intensity exhibited 40-fold enhancement at 511 nm. In addition, the probe showed excellent selectivity, high sensitivity, fast responds and low limit detection for palladium with a larger Stoke-shift. Moreover, the targetable probe was also successfully applied for detecting palladium in lysosomes of living cells. Hence, the probe though ESIPT modulation is a promising for monitoring palladium in practical samples.
一种新型的溶酶体靶向邻苯二甲酰亚胺荧光探针被设计用于基于 ESIPT 用于信号转导的钯检测。由于 ESIPT 过程受到丙烯酰胺的阻碍，与丙烯酰胺偶联的荧光探针显示出较弱的荧光。但是，随着钯的加入，通过钯催化的去烯丙基化反应恢复了 ESIPT 发射，并且荧光强度在 511nm 处显示出 40 倍的增强。此外，该探针对钯具有优异的选择性、高灵敏度、快速响应和低检测限，具有较大的斯托克斯位移。此外，靶向探针还成功地应用于活细胞溶酶体中钯的检测。因此，该探针通过 ESIPT 调制有望用于实际样品中钯的监测。
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