School of Pharmaceutical Sciences, Jining Medical University, Rizhao, 276826, China.
State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, China.
Anal Sci. 2022 Apr;38(4):689-694. doi: 10.1007/s44211-022-00084-9. Epub 2022 Mar 2.
A novel fluorescence-enhanced probe 2-butyl-1,3-dioxoisoindolin-4-yl picolinate (BDIP) has been developed for the detection of Cu based on the excited-state intramolecular proton transfer (ESIPT) process. BDIP utilized a phthalimide derivative as the fluorophore and selected picolinate ester as the recognition site for Cu. The probe displayed high selectivity, strong anti-interference ability, and a significant fluorescence enhancement effect for Cu in phosphate buffer saline (PBS, 10 mM, pH 7.4) with the detection limit of 31 nM. BDIP also possesses the advantages of simple synthesis steps, large Stokes shift, and good water solubility. Moreover, BDIP was used for Cu detection in real water samples, with the result being satisfactory.
一种新型荧光增强探针 2-丁基-1,3-二氧代异吲哚啉-4-基吡啶甲酸酯（BDIP）已被开发用于基于激发态分子内质子转移（ESIPT）过程的 Cu 检测。BDIP 利用邻苯二甲酰亚胺衍生物作为荧光团，并选择吡啶甲酸酯作为 Cu 的识别位点。探针在磷酸盐缓冲盐水（PBS，10 mM，pH 7.4）中对 Cu 表现出高选择性、强抗干扰能力和显著的荧光增强效应，检测限为 31 nM。BDIP 还具有合成步骤简单、Stokes 位移大、水溶性好等优点。此外，BDIP 还用于实际水样中的 Cu 检测，结果令人满意。
