Maglione Michele, Salvador Enrico, Ruaro Maria E, Melato Mauro, Tromba Giuliana, Angerame Daniele, Bevilacqua Lorenzo
Department of Medical Sciences, University of Trieste, Trieste 34125, Italy.
SISSA-International School for Advanced Studies, Trieste 34136, Italy.
J Biomed Res. 2018 Jul 11;33(1):38-45. doi: 10.7555/JBR.32.20160066.
It has been shown that stem cells are able to calcify both in vitro and in vivo once implanted under the skin, if conveniently differentiated. Nowadays, however, a study on their efficiency in osseous regeneration does not exist in scientific literature and this very task is the real aim of the present experimentation. Five different defects of 6 mm in diameter and 2 mm in depth were created in the calvaria of 8 white New Zealand rabbits. Four defects were regenerated using 2 different conveniently modified scaffolds (Bio-Oss® Block and Bio-Oss Collagen®, Geistlich), with and without the aid of stem cells. After the insertion, the part was covered with a collagen membrane fixed by 5 modified titan pins (Altapin®). The defect in the front was left empty on purpose as an internal control to each animal. Two animals were sacrificed respectively after 2, 4, 6, 10 weeks. The samples were evaluated with micro-CT and histological analysis. Micro-CT analysis revealed that the quantity of new bone for samples with Bio-Oss® Block and stem cells was higher than for samples with Bio-Oss® Block alone. Histological analysis showed that regeneration occurred in an optimal way in every sample treated with scaffolds. The findings indicated that the use of adult stem cells combined with scaffolds accelerated some steps in normal osseous regeneration.
研究表明，干细胞一旦皮下植入并方便地分化后，在体外和体内均能钙化。然而，目前科学文献中尚无关于其骨再生效率的研究，而本实验的真正目的正是此项任务。在8只白色新西兰兔的颅骨上制造了5个直径6毫米、深2毫米的不同缺损。使用2种不同的经适当改良的支架（Geistlich公司的Bio-Oss® 块状骨和Bio-Oss胶原骨），在有和没有干细胞辅助的情况下，对4个缺损进行再生处理。植入后，该部位用5枚改良钛钉（Altapin®）固定的胶原膜覆盖。每只动物前方的缺损特意留空作为内部对照。分别在2、4、6、10周后处死2只动物。对样本进行了显微CT和组织学分析。显微CT分析显示，使用Bio-Oss® 块状骨和干细胞的样本的新骨量高于仅使用Bio-Oss® 块状骨的样本。组织学分析表明，用支架处理的每个样本均以最佳方式发生了再生。研究结果表明，成年干细胞与支架联合使用加速了正常骨再生的某些步骤。
