Puzyr A P, Medvedeva S E, Burov A E, Zernov Yu P, Bondar V S
Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russia.
Institute of Computational Technologies, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660049, Russia.
Dokl Biochem Biophys. 2018 May;480(1):173-176. doi: 10.1134/S1607672918030146. Epub 2018 Jul 14.
In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10-1.4 × 10 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.
