松蕈酸和月桃生物活性成分对3T3-L1脂肪细胞的抗肥胖作用。
Anti-obesity effects of hispidin and Alpinia zerumbet bioactives in 3T3-L1 adipocytes.
作者信息
Tu Pham Thi Be, Tawata Shinkichi
机构信息
Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-8580, Japan.
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0129, Japan.
出版信息
Molecules. 2014 Oct 15;19(10):16656-71. doi: 10.3390/molecules191016656.
Obesity and its related disorders have become leading metabolic diseases. In the present study, we used 3T3-L1 adipocytes to investigate the anti-obesity activity of hispidin and two related compounds that were isolated from Alpinia zerumbet (alpinia) rhizomes. The results showed that hispidin, dihydro-5,6-dehydrokawain (DDK), and 5,6-dehydrokawain (DK) have promising anti-obesity properties. In particular, all three compounds significantly increased intracellular cyclic adenosine monophosphate (cAMP) concentrations by 81.2% ± 0.06%, 67.0% ± 1.62%, and 56.9% ± 0.19%, respectively. Hispidin also stimulated glycerol release by 276.4% ± 0.8% and inhibited lipid accumulation by 47.8% ± 0.16%. Hispidin and DDK decreased intracellular triglyceride content by 79.5% ± 1.37% and 70.2% ± 1.4%, respectively, and all three compounds inhibited glycerol-3-phosphate dehydrogenase (GPDH) and pancreatic lipase, with hispidin and DDK being the most potent inhibitors. Finally, none of the three compounds reduced 3T3-L1 adipocyte viability. These results highlight the potential for developing hispidin and its derivatives as anti-obesity compounds.
肥胖及其相关疾病已成为主要的代谢性疾病。在本研究中，我们使用3T3-L1脂肪细胞来研究从艳山姜根茎中分离出的苔黑酚葡萄糖苷及其两种相关化合物的抗肥胖活性。结果表明，苔黑酚葡萄糖苷、二氢-5,6-脱氢卡瓦因（DDK）和5,6-脱氢卡瓦因（DK）具有良好的抗肥胖特性。特别是，这三种化合物分别使细胞内环磷酸腺苷（cAMP）浓度显著增加了81.2%±0.06%、67.0%±1.62%和56.9%±0.19%。苔黑酚葡萄糖苷还使甘油释放量增加了276.4%±0.8%，并使脂质积累减少了47.8%±0.16%。苔黑酚葡萄糖苷和DDK分别使细胞内甘油三酯含量降低了79.5%±1.37%和70.2%±1.4%，并且这三种化合物均抑制了3-磷酸甘油脱氢酶（GPDH）和胰脂肪酶，其中苔黑酚葡萄糖苷和DDK是最有效的抑制剂。最后，这三种化合物均未降低3T3-L1脂肪细胞的活力。这些结果突出了将苔黑酚葡萄糖苷及其衍生物开发为抗肥胖化合物的潜力。
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