Zhang Zhanhe, Wu Donglin, Li Xintong, Jia Yang, Qi Jingwei, Harmon David L, Xu Ming
College of Animal Science, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, Hohhot, 010018, China.
Trop Anim Health Prod. 2025 May 29;57(5):234. doi: 10.1007/s11250-025-04464-x.
The effects of sodium butyrate (SB) supplementation in whole milk (WM), and/or a starter mixture (SM) on the growth performance, selected blood parameters, gastrointestinal tract (GIT) development, and tight junction (TJ) proteins of the gastrointestinal tract in preweaned calves were determined. Eighty calves, averaging of 3 (± 0.74) d old, were randomly allocated into 1 of 4 groups (n = 20 per group) and were fed (1) WM and SM without SB (control group); (2) SM supplemented with SB (0.3% of dry matter basis; SM group); (3) WM supplemented with SB (WM, 8.8 g/d) (WM group); or (4) WM and SM supplemented with SB (SMWM group). At d 74, four calves from each group were euthanized for in-depth analysis. The addition of SB to the SM increased the ADG and reduced fecal scores throughout the preweaned period (P < 0.05). SB supplementation in WM notably lowered diarrhea incidence throughout the preweaned period (P < 0.05). The addition of SB to the SM increased blood beta-hydroxybutyric acid and diamine oxidase concentrations at d 24 and insulin-like growth factor 1 concentrations at d 44 (P < 0.05). SB addition to WM prominently enhanced rumen papilla dimensions but reduced jejunum crypt depth alongside villus height and depth (P < 0.05). The addition of SB to the SM diminished the mucous layer thickness of the rumen and increased TJ protein expression in the GIT (P < 0.05). Based on these results, we found that the addition of SB to the SM and WM had a positive effect on calf general health, blood indexes, and GIT development. The addition of SB to both WM and SM could be recommended for preweaned calves.
本研究测定了在全脂牛奶（WM）中添加丁酸钠（SB）和/或起始混合物（SM）对断奶前犊牛生长性能、选定血液参数、胃肠道（GIT）发育以及胃肠道紧密连接（TJ）蛋白的影响。80头平均3（±0.74）日龄的犊牛被随机分为4组，每组20头，并分别饲喂：（1）不添加SB的WM和SM（对照组）；（2）添加SB的SM（干物质基础的0.3%；SM组）；（3）添加SB的WM（WM，8.8 g/d）（WM组）；或（4）添加SB的WM和SM（SMWM组）。在第74天，每组选取4头犊牛实施安乐死以进行深入分析。在SM中添加SB可提高断奶前犊牛的平均日增重（ADG）并降低粪便评分（P < 0.05）。在WM中添加SB可显著降低断奶前犊牛腹泻的发生率（P < 0.05）。在SM中添加SB可使第24天血液中β-羟基丁酸和二胺氧化酶浓度升高，并使第44天胰岛素样生长因子1浓度升高（P < 0.05）。在WM中添加SB可显著增加瘤胃乳头尺寸，但同时降低空肠隐窝深度以及绒毛高度和深度（P < 0.05）。在SM中添加SB可减少瘤胃黏液层厚度并增加GIT中TJ蛋白的表达（P < 0.05）。基于这些结果，我们发现向SM和WM中添加SB对犊牛的总体健康、血液指标和GIT发育具有积极影响。建议在断奶前犊牛的WM和SM中均添加SB。
