Brykov V A, Kukhlevskiĭ A D
Mol Biol (Mosk). 1988 Mar-Apr;22(2):377-83.
Inverted repeat DNA sequences during embryogenesis were tested by comparing the bulk inverted repeat taken from Strongylocentrotus intermedius sea urchin embryos at different stages of development. This fraction exhibited quantitative and qualitative changes. A reversible quantitative decrease was associated with the 16-cell embryo and blastula stages. Sizing on 1.5% agarose gel indicated that the length of the palindromic sequences at the early blastula stage was predominantly about 200 b. p., and at the pluteus stage 240 b. p. Sensitivity of the palindromic sequences to S1 nuclease digestion at the blastula and gastrula stages was different. It was shown that a specific set of the inverted repeats was included in fragments of DNA--comparising the origin of replication. The results suggest that the change of inverted repeats may be determined by replication processes.
通过比较取自中间球海胆胚胎不同发育阶段的大量反向重复序列，对胚胎发育过程中的反向重复DNA序列进行了测试。这一部分呈现出数量和质量上的变化。16细胞胚胎期和囊胚期出现了可逆的数量减少。在1.5%琼脂糖凝胶上进行大小测定表明，囊胚早期回文序列的长度主要约为200碱基对，而在长腕幼虫期为240碱基对。囊胚期和原肠胚期回文序列对S1核酸酶消化的敏感性不同。结果表明，一组特定的反向重复序列包含在DNA片段中——比较复制起点。结果表明，反向重复序列的变化可能由复制过程决定。
