Toivanen A, Toivanen P
J Immunol. 1977 Feb;118(2):431-6.
Cyclophosphamide-treated newly hatched chicks were transplanted with histocompatible, semiallogeneic and allogeneic combinations of B (bursa) and T (thymus) cells from newly hatched donors. At the age of 5 weeks the birds were studied for an anti-SRBC response and for the generation of germinal centers in the spleen. The results of these experiments are summarized as follows. i) Allogeneic bursal stem cells have the capacity to restore the bursal structures of CY-treated recipients, but not the germinal center or anti-SRBC formation. ii) When allogeneic B cells are combined with T cells histocompatible or semiallogeneic with them, a restoration of the germinal center formation is achieved, but not to the same level as observed in normal birds or in CY-treated birds transplanted with histocompatible or semiallogeneic B cells. iii) Allogeneic B cells, even when complemented with T cells histocompatible with them, fail to restore the antibody production against SRBC; this is achieved only after transplantation of B cells histocompatible or semiallogeneic with the recipient. These findings indicate that germinal center formation is dependent on cooperation of histocompatible or semiallogeneic B and T cells, and furthermore, that an additional factor provided by the host is involved. Studies with transplantation of histocompatible and histoincompatible 'empty' splenic stromata revealed that the additional factor is not related to the splenic stroma.
用环磷酰胺处理刚孵出的雏鸡，然后将来自刚孵出的供体的B（法氏囊）细胞和T（胸腺）细胞的组织相容性、半同种异体和同种异体组合移植到雏鸡体内。在5周龄时，对这些鸡进行抗绵羊红细胞（SRBC）反应以及脾脏生发中心生成情况的研究。这些实验结果总结如下：i）同种异体法氏囊干细胞有能力恢复经环磷酰胺处理的受体的法氏囊结构，但不能恢复生发中心或抗SRBC的形成。ii）当同种异体B细胞与与其组织相容性或半同种异体的T细胞组合时，生发中心的形成得以恢复，但恢复程度不如正常鸡或移植了组织相容性或半同种异体B细胞的经环磷酰胺处理的鸡。iii）同种异体B细胞，即使与与其组织相容性的T细胞互补，也无法恢复针对SRBC的抗体产生；只有在移植与受体组织相容性或半同种异体的B细胞后才能实现抗体产生。这些发现表明，生发中心的形成依赖于组织相容性或半同种异体的B细胞和T细胞的合作，此外，还涉及宿主提供的另一个因素。对组织相容性和组织不相容的“空”脾基质移植的研究表明，这个额外因素与脾基质无关。
