LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53.

BACKGROUND

Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown.

METHODS

We knocked down and overexpressed in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of .

RESULTS

Knockdown of significantly inhibited OS cell viability and promoted apoptosis. , which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of . Overexpression of significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of partially suppressed the increased OS cell apoptosis induced by the inhibition of , suggesting that is a key functional downstream gene of .

CONCLUSIONS

Our findings suggest that promotes OS cell viability and survival by regulating expression.