Liu Qinhua, Lindow Steven E, Zhang Jianguo
South Pratacultural Research Center, South China Agricultural University, Guangzhou, China.
Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
Anim Sci J. 2018 Sep;89(9):1302-1309. doi: 10.1111/asj.13063. Epub 2018 Jul 15.
Enhancing the aerobic stability of silage is very important in silage production. The objective of this study was to compare the roles of a new bacterial strain Lactobacillus parafarraginis ZH1 with Lactobacillus plantarum Chikuso-1 (LP) and Lactobacillus buchneri NCIMB 40788 (LB) in improving the aerobic stability of oat silage and identify the anti-yeast substances produced by them. After ensiled for 45 days, either inoculated silages or control silage were of pH values lower than 4.2. The control and LP inoculated silage had poorer aerobic stability than LB and ZH1 inoculated ones (p < 0.05). ZH1 inoculated silage produced more acetic acid, benzoic acid and hexadecanoic acid than LB inoculated one and had the best aerobic stability (p < 0.05). In MRS medium, strain ZH1 produced more hexadecanoic acid than LP and LB strains, more benzoic acid than strain LP (p < 0.05), and the equal amount of benzoic acid to LB (p > 0.05). In addition, benzoic acid and hexadecanoic acid had low minimal inhibitory concentrations to target yeasts in the pure culture. In conclusion, L. parafarraginis ZH1 had stronger anti-yeast potential and more effectively improved the aerobic stability of silage than other strains used in this study.
提高青贮饲料的有氧稳定性在青贮饲料生产中非常重要。本研究的目的是比较新型菌株副干酪乳杆菌ZH1与植物乳杆菌Chikuso-1（LP）和布氏乳杆菌NCIMB 40788（LB）在提高燕麦青贮饲料有氧稳定性方面的作用，并鉴定它们产生的抗酵母物质。青贮45天后，接种青贮饲料和对照青贮饲料的pH值均低于4.2。对照和接种LP的青贮饲料的有氧稳定性比接种LB和ZH1的青贮饲料差（p<0.05）。接种ZH1的青贮饲料比接种LB的青贮饲料产生更多的乙酸、苯甲酸和十六烷酸，且有氧稳定性最佳（p<0.05）。在MRS培养基中，ZH1菌株产生的十六烷酸比LP和LB菌株多，产生的苯甲酸比LP菌株多（p<0.05），产生的苯甲酸与LB菌株等量（p>0.05）。此外，苯甲酸和十六烷酸对纯培养中的目标酵母具有较低的最低抑菌浓度。总之，副干酪乳杆菌ZH1比本研究中使用的其他菌株具有更强的抗酵母潜力，能更有效地提高青贮饲料的有氧稳定性。
