Liu Q H, Yang F Y, Zhang J G, Shao T
College of Agriculture, South China Agricultural University, Guangzhou, China; College of Prataculture Science, Nanjing Agricultural University, Nanjing, China.
J Appl Microbiol. 2014 Aug;117(2):405-16. doi: 10.1111/jam.12530. Epub 2014 May 21.
Lactobacillus parafarraginis ZH1 isolated from silage was characterized, and the effects of inoculating ZH1 and Lact. buchneri (LB) on the aerobic stability of sweet corn stalk (SCS) silage and whole-plant oat (WPO) silage ensiled at 15 and 30°C were studied.
After ensiling of SCS or WPO in plastic bottle silo for 45 days, silos were opened, and aerobic stability was studied by monitoring temperature change with thermo recorders in silage for 6 days. SCS silage and WPO silage were well conserved naturally at both storage temperatures. However, silages were prone to aerobic deterioration due to the presence of residual yeasts. ZH1 inoculated silages ensiled at both temperatures, LB inoculated silages ensiled at 30°C had better aerobic stability than the uninoculated silages and the LB-inoculated silage at 15°C.
Strain ZH1 improved the aerobic stability of SCS silage and WPO silage ensiled at both 15 and 30°C, while LB improved the aerobic stability of silage only ensiled at the high temperature of 30°C.
The new strain ZH1 can be used as an effective inhibitor for aerobic deterioration of silage maintained from 15 to 30°C.
对从青贮饲料中分离出的副干酪乳杆菌ZH1进行特性鉴定，并研究接种ZH1和布氏乳杆菌（LB）对在15℃和30℃条件下青贮的甜玉米秸秆（SCS）青贮饲料和全株燕麦（WPO）青贮饲料有氧稳定性的影响。
将SCS或WPO在塑料瓶青贮窖中青贮45天后打开青贮窖，通过用温度记录仪监测青贮饲料中的温度变化6天来研究有氧稳定性。SCS青贮饲料和WPO青贮饲料在两个储存温度下均自然保存良好。然而，由于残留酵母的存在，青贮饲料容易发生有氧变质。在两个温度下接种ZH1的青贮饲料，在30℃下接种LB的青贮饲料比未接种的青贮饲料以及在15℃下接种LB的青贮饲料具有更好的有氧稳定性。
菌株ZH1提高了在15℃和30℃下青贮的SCS青贮饲料和WPO青贮饲料的有氧稳定性，而LB仅提高了在30℃高温下青贮的青贮饲料的有氧稳定性。
新菌株ZH1可作为15℃至30℃保存的青贮饲料有氧变质的有效抑制剂。
