Departamento de Producción Vegetal, Universidad Politécnica de Cartagena, Cartagena, Spain.
Bayer Crop Science S.L, Development Iberia, Paterna, Spain.
Pest Manag Sci. 2019 Jan;75(1):45-52. doi: 10.1002/ps.5144. Epub 2018 Sep 11.
Spiromesifen and spirotetramat are novel insecticides belonging to the chemical class of tetronic and tetramic acid derivatives. Both compounds have proven very effective against field populations of Bemisia tabaci around the world. However, several growers have recently reported control failures in Spain. Therefore, we studied the resistance level to these insecticides in field populations reporting control failures. In addition, we further selected a spiromesifen-resistant strain to study the mechanisms involved and the cross-resistance pattern.
All the new field populations collected were significantly more resistant to spiromesifen than the susceptible population, confirming the presence of resistance. Several populations showing high levels of resistance to spiromesifen (>10 000-fold), exhibited cross-resistance to spirotetramat, but resistance ratios were much lower (130-fold). The spiromesifen laboratory-selected strain was very resistant to spiromesifen (LC > 30 000 mg L ) and spirotetramat (LC = 368.1 mg L ), but lacks any cross-resistance to other insecticides, thus providing options for resistance management. None of the synergists tested significantly restored the susceptibility of B. tabaci to either spiromesifen or spirotetramat.
This is the first report of resistance to spiromesifen and spirotetramat in B. tabaci, and such high levels of resistance have not been reported before in any field collected pest. Our results suggest that enhanced detoxification does not critically contribute to resistance to ketoenols in B. tabaci. The obvious lack of a metabolic resistance mechanism either suggests a target-site resistance mechanism or a metabolic mechanism insensitive to the synergists tested. © 2018 Society of Chemical Industry.
螺虫乙酯和螺甲螨酯是两种新型杀虫剂，属于四氢呋喃和四氢噻喃酸衍生物化学类别。这两种化合物在世界范围内对烟粉虱田间种群都非常有效。然而，最近一些种植者报告在西班牙防治失败。因此，我们研究了报告防治失败的田间种群对这些杀虫剂的抗性水平。此外，我们进一步选择了螺虫乙酯抗性菌株，以研究相关机制和交叉抗性模式。
所有新收集的田间种群对螺虫乙酯的抗性明显高于敏感种群，证实了抗性的存在。一些对螺虫乙酯表现出高抗性水平（>10 000 倍）的种群对螺甲螨酯表现出交叉抗性，但抗性倍数要低得多（130 倍）。螺虫乙酯实验室选择的菌株对螺虫乙酯（LC > 30 000 mg/L）和螺甲螨酯（LC = 368.1 mg/L）非常具有抗性，但对其他杀虫剂没有任何交叉抗性，因此为抗性管理提供了选择。测试的增效剂均不能显著提高烟粉虱对螺虫乙酯或螺甲螨酯的敏感性。
这是首次报道烟粉虱对螺虫乙酯和螺甲螨酯的抗性，在任何田间采集的害虫中，以前都没有报道过如此高的抗性水平。我们的研究结果表明，解毒增强并没有对烟粉虱对酮烯醇的抗性起到关键作用。明显缺乏代谢抗性机制，这要么暗示了靶标抗性机制，要么暗示了对测试增效剂不敏感的代谢机制。 © 2018 英国化学学会。
