College of Plant Science, Jilin University, Changchun 130062, China.
School of Agricultural Science, Zhengzhou University, Zhengzhou 450001, China.
Int J Mol Sci. 2022 Feb 21;23(4):2356. doi: 10.3390/ijms23042356.
Chemosensory proteins (CSPs) are a class of transporters in arthropods. Deeper research on CSPs showed that CSPs may be involved in some physiological processes beyond chemoreception, such as insect resistance to pesticides. We identified two upregulated in two resistant strains of Glover. To understand their role in the resistance of aphids to pesticides, we performed the functional verification of and in vivo and in vitro. Results showed that the sensitivity of the thiamethoxam-resistant strain to thiamethoxam increased significantly with the silencing of and by RNAi (RNA interference), and the sensitivity of the spirotetramat-resistant strain to spirotetramat increased significantly with the silencing of . Transgenic expressing exhibited stronger resistance to thiamethoxam, spirotetramat, and alpha-cypermethrin than the control did. In the bioassay of transgenic , showed different tolerance mechanisms for different pesticides, and the overexpressed may play a role in processes other than resistance to pesticides. In brief, the present results prove that are related to the resistance of cotton aphids to insecticides.
化感蛋白（CSPs）是节肢动物中的一类转运蛋白。对 CSPs 的深入研究表明，CSPs 可能参与了除化学感受以外的一些生理过程，例如昆虫对杀虫剂的抗性。我们在两种 Glover 抗性品系中鉴定出两个上调的 。为了了解它们在蚜虫对杀虫剂抗性中的作用，我们在体内和体外对 和 进行了功能验证。结果表明，用 RNAi（RNA 干扰）沉默 和 后，噻虫嗪抗性品系对噻虫嗪的敏感性显著增加，而螺虫乙酯抗性品系对螺虫乙酯的敏感性显著增加。表达 的转基因 对噻虫嗪、螺虫乙酯和高效氯氰菊酯的抗性均强于对照。在转基因 的生物测定中， 对不同杀虫剂表现出不同的耐受机制，过表达的 可能在杀虫剂抗性以外的过程中发挥作用。总之，本研究结果证明 与棉蚜对杀虫剂的抗性有关。
