Chemistry Department, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Indonesia.
Chemistry Department, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Indonesia.
Int J Biol Macromol. 2018 Nov;119:166-171. doi: 10.1016/j.ijbiomac.2018.07.038. Epub 2018 Jul 26.
Chitosan is a biopolymer that has antifungal activity against C. albicans. Chemical modification of chitosan can provide it with new functional properties for a wide range of biological and biomedical applications. Carboxymethyl chitosan is a derivative of chitosan obtained by etherification of alkaline chitosan with monochloroacetic acid. Carboxymethyl chitosan has a higher solubility than chitosan; therefore it is more readily applicable for use in various fields. Chitosan also has antifungal activity against C. albicans. This study evaluated carboxymethyl chitosan as a gauze-coating material to be used for its antifungal properties. This study also optimized the coating process. Gauze was coated with carboxymethyl chitosan then characterized by Fourier Transform Infra-Red Spectrophotometer (FTIR), X-ray diffraction and scanning electron microscopy (SEM). The antifungal activities of gauze-coated samples were then tested by the diffusion method. The results show that the optimum conditions for the process of coating gauze with carboxymethyl chitosan are dipping ten times at a concentration of 1% for 50 s. Antifungal activities of carboxymethyl chitosan-coated gauze as measured by the diameter of the growth inhibition area are 0.30 cm higher than chitosan-coated gauze, which has a growth-inhibition diameter of 0.12 cm.
壳聚糖是一种具有抗白色念珠菌活性的生物聚合物。壳聚糖的化学修饰可以为其提供新的功能特性，从而广泛应用于生物和生物医学领域。羧甲基壳聚糖是壳聚糖的一种衍生物，通过碱性壳聚糖与一氯乙酸的醚化反应获得。羧甲基壳聚糖的溶解度高于壳聚糖，因此更易于在各种领域应用。壳聚糖对白色念珠菌也具有抗真菌活性。本研究评估了羧甲基壳聚糖作为一种纱布涂层材料，以利用其抗真菌特性。本研究还对涂层工艺进行了优化。将羧甲基壳聚糖涂覆在纱布上，然后通过傅里叶变换红外光谱仪（FTIR）、X 射线衍射和扫描电子显微镜（SEM）进行表征。然后通过扩散法测试纱布涂层样品的抗真菌活性。结果表明，用羧甲基壳聚糖涂覆纱布的最佳工艺条件是在 1%浓度下浸泡 10 次，每次 50 秒。羧甲基壳聚糖涂层纱布的抗真菌活性，以抑菌区的生长抑制直径来衡量，比壳聚糖涂层纱布高 0.30cm，壳聚糖涂层纱布的生长抑制直径为 0.12cm。
