PhD. Universidade Federal de Goiás - Instituto de Patologia Tropical e Saúde Pública - Programa de Pós-Graduação em Medicina Tropical e Saúde Pública - Goiânia (GO), Brazil.
PhD. Universidade Estadual de Goiás - Campus de Ciências Exatas e Tecnológicas - Anápolis (GO), Brazil.
Acta Cir Bras. 2021 Apr 5;36(3):e360303. doi: 10.1590/ACB360303. eCollection 2021.
This study aimed to elaborate a hydrogel constituted by carboxymethyl chitosan (CMC), hyaluronic acid (HA) and silver (Ag) and to evaluate its healing effect on partial-thickness burn wounds experimentally induced in rats.
CMC was obtained by chitosan reacting with monochloroacetic acid. The carboxymethylation was confirmed by Fourier-transform infrared spectroscopy and hydrogen nuclear magnetic resonance (NMR). Scanning electron microscopy was used to determine the morphologicalcharacteristics of chitosan and CMC. After the experimental burn wound induction, the animals (n = 126) were treated with different CMC formulations, had their occlusive dressings changed daily and were followed through 7, 14 and 30 days. Morphometric, macroscopic and microscopic aspects and collagen quantification were evaluated.
Significative wound contraction, granulation tissue formation, inflammatory infiltration and collagen fibers deposit throughout different phases of the healing process were observed in the CMC hydrogels treated groups.
The results showed that, in the initial phase of the healing process, the most adequate product was the CMC/HA/Ag association, while in the other phases the CMC/HA association was the best one to promote the healing of burn wounds.
本研究旨在阐述一种由羧甲基壳聚糖（CMC）、透明质酸（HA）和银（Ag）组成的水凝胶，并评估其在大鼠实验性部分厚度烧伤创面的愈合效果。
通过壳聚糖与一氯乙酸反应获得 CMC。通过傅里叶变换红外光谱和氢核磁共振（NMR）确认羧甲基化。扫描电子显微镜用于确定壳聚糖和 CMC 的形态特征。在诱导实验性烧伤创面后，将动物（n=126）用不同的 CMC 制剂进行处理，每日更换密闭敷料，并进行 7、14 和 30 天的随访。评估形态学、宏观和微观方面以及胶原蛋白定量。
在 CMC 水凝胶处理组中，观察到明显的创面收缩、肉芽组织形成、炎症浸润和胶原纤维沉积，贯穿愈合过程的不同阶段。
结果表明，在愈合过程的初始阶段，最合适的产品是 CMC/HA/Ag 联合物，而在其他阶段，CMC/HA 联合物是促进烧伤创面愈合的最佳选择。
