Mosquito, Microbiology and Nanotechnology Research Units, Parasitology Laboratory, Department of Zoology, The University of Burdwan, Burdwan-713104, West Bengal, India.
Curr Drug Discov Technol. 2021;18(4):503-510. doi: 10.2174/1570163817666200727101300.
The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin.
The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica.
Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically.
Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract, five different bioactive compounds e.g., 2,4-ditert -butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene- 2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified.
Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.
解决抗生素耐药性日益严重问题的唯一方法是发现具有天然来源的抗菌剂。
本研究旨在寻找鸡蛋花成熟叶片的粗提物和溶剂提取物的抗菌潜力。
通过测量抑菌圈并确定最小抑菌浓度和最小杀菌浓度值，评估了 3 种不同溶剂提取物对 4 种人类和 4 种鱼类致病菌的抗菌活性。标准抗生素用作阳性对照。对最有效的提取物（乙酸乙酯提取物）进行了初步的植物化学筛选，对乙酸乙酯提取物的傅里叶变换红外分析和薄层色谱（TLC）馏分的气相色谱-质谱分析进行了细致的研究。所有实验均进行了 3 次，并进行了统计学分析。
粗提物和溶剂提取物均能有效抑制所选细菌的生长。乙酸乙酯提取物在所有测试菌株中均表现出最高的抑制圈，对大肠杆菌（MTCC 739）的最大抑制率为 19.50±0.29mm。提取物的 MBC/MIC 表明，所有 3 种溶剂提取物均具有杀菌作用。初步的植物化学测试显示存在单宁、甾体和生物碱，傅里叶变换红外分析显示存在许多功能团，如醇、酰胺、胺盐和醛基。从乙酸乙酯提取物 TLC 馏分的气相色谱-质谱分析中，鉴定出 5 种不同的生物活性化合物，如 2,4-二叔丁基苯基 5-羟基戊酸酯、草酸；烯丙基壬基酯、7,9-二叔丁基-1-氧杂螺（4,5）癸-6,9-二烯-2,8-二酮、邻苯二甲酸二丁酯和 2,3,5,8-四甲基-十二烷。
鸡蛋花叶片提取物含有可作为广谱杀菌剂的生物活性化合物。
