State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Chem Commun (Camb). 2018 Aug 11;54(62):8594-8597. doi: 10.1039/c8cc04215b. Epub 2018 Jul 16.
The regiospecific glycosylation of pentacyclic triterpenoids by UGT73F17, a new glycosyltransferase from Glycyrrhiza uralensis, is highlighted. UGT73F17 exhibited strict substrate specificity toward the carboxyl group at C-30/C-29 of pentacyclic triterpenoids, and showed high promiscuity to sugar donors. UGT73F17 represents the first identified triterpenoid 30/29-O-glycosyltransferase, and could be used as an effective biocatalyst to synthesize glycosyl ester saponins.
本研究强调了来源于甘草的新型糖基转移酶 UGT73F17 对五环三萜的区域选择性糖基化。UGT73F17 对五环三萜 C-30/C-29 位羧酸基团具有严格的底物特异性，同时对糖供体表现出高度的混杂性。UGT73F17 代表了首个鉴定的三萜 30/29-O-糖基转移酶，可作为有效生物催化剂用于合成糖基酯皂苷。
