克隆的单一DNA在19号染色体三个不同区域的定位：强直性肌营养不良连锁探针的筛选
Localization of cloned unique DNA to three different regions of chromosome 19: screen for linkage probes for myotonic dystrophy.
作者信息
Yamaoka L H, Bartlett R J, Ross D A, Fey G H, Ledbetter D H, Bruns G, Pericak-Vance M A, Herbstreith M H, Roses A D
出版信息
J Neurogenet. 1985 Dec;2(6):403-12. doi: 10.3109/01677068509101426.
Screening polymorphic DNA probes for linkage to myotonic dystrophy (DM) and to other reported chromosome 19 (CH19) genes will develop a linkage map for human CH19. We report here the assignment of 3 cloned unique DNA sequences to 3 distinct regions of CH19. The novel use of 35S-labeled probes facilitated the rapid localization of the gene for the third complement factor (C3) to 19p13.2 by in situ hybridization. Metaphase chromosomes were from normal peripheral lymphocytes as well as from a fibroblast line containing a 15;19 translocation which permitted clear identification of CH19 regions of localization. Two random clones isolated from a plasmid library of human F-group enriched chromosomal DNA (D19S5 and D19S6) were in like manner assigned to 19p1.2 and 19q13.2 to 19qter, respectively.
筛选与强直性肌营养不良（DM）以及其他已报道的19号染色体（CH19）基因连锁的多态性DNA探针，将绘制出人类CH19的连锁图谱。我们在此报告3个克隆的独特DNA序列定位于CH19的3个不同区域。35S标记探针的新应用通过原位杂交促进了第三补体因子（C3）基因快速定位于19p13.2。中期染色体来自正常外周淋巴细胞以及含有15;19易位的成纤维细胞系，这使得能够清晰鉴定定位的CH19区域。从富含人类F组染色体DNA的质粒文库中分离出的两个随机克隆（D19S5和D19S6），同样分别定位于19p1.2和19q13.2至19qter。
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