IVF Australia, Sydney, New South Wales, Australia.
Fertil Steril. 2011 Aug;96(2):389-93. doi: 10.1016/j.fertnstert.2011.05.023. Epub 2011 Jun 8.
To investigate the relationship between spindle normality and: 1) blastocyst formation; 2) implantation rates; and 3) live birth rates.
Prospective observational study.
A private fertility clinic.
PATIENT(S): One hundred patients ≤40 years old undergoing intracytoplasmic sperm injection (ICSI).
INTERVENTION(S): Meiotic spindles were imaged before ICSI with the use of Oosight microscopy. With the use of specific criteria, spindle normality was independently assessed by two researchers. Blastocysts were chosen for transfer by standard light microscopic morphologic criteria by researchers who were blinded to the spindle assessment.
MAIN OUTCOME MEASURE(S): 1) Blastocyst formation; 2) implantation; and 3) live birth rates.
RESULT(S): A total of 808/920 oocytes were metaphase II. Of those, 711 (88%) had a visible spindle: 205 (29%) were normal spindles (NS) and 506 (71%) abnormal spindles (AS). Fertilization rates were significantly higher in NS oocytes. Although NS and AS oocytes both formed morphologically good-quality blastocysts, implantation and live birth rates were higher from NS oocytes. All ongoing pregnancies resulted from NS oocytes.
CONCLUSION(S): Spindle assessment with polarized light microscopy provides an early predictor of the pregnancy potential of that oocyte. AS oocytes can form good-quality blastocysts, but these appear to have little chance of live birth. Therefore, spindle assessment should improve the selection of the best embryo for single embryo transfer.
研究纺锤体正常与以下因素的关系：1）囊胚形成；2）着床率；3）活产率。
前瞻性观察研究。
一家私人生育诊所。
100 名年龄≤40 岁接受卵胞浆内单精子注射（ICSI）的患者。
在 ICSI 前使用 Oosight 显微镜对减数分裂纺锤体进行成像。根据特定标准，由两位研究人员独立评估纺锤体的正常情况。通过研究人员使用标准的光镜形态学标准选择用于转移的囊胚，这些研究人员对纺锤体评估是盲法的。
1）囊胚形成；2）着床；3）活产率。
共有 808/920 个卵母细胞处于 MII 期。其中，711 个（88%）有可见纺锤体：205 个（29%）为正常纺锤体（NS），506 个（71%）为异常纺锤体（AS）。NS 卵母细胞的受精率显著更高。尽管 NS 和 AS 卵母细胞均形成形态良好的优质囊胚，但 NS 卵母细胞的着床率和活产率更高。所有持续妊娠均来自 NS 卵母细胞。
偏振光显微镜下的纺锤体评估为该卵母细胞的妊娠潜力提供了早期预测。AS 卵母细胞可以形成优质囊胚，但这些囊胚似乎几乎没有活产的机会。因此，纺锤体评估应提高用于单胚胎移植的最佳胚胎的选择。
