Lin Jianfeng, Chen Youqin, Liu Liya, Shen Aling, Zheng Wei
Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China.
Pediatric Department, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Oncol Lett. 2018 Aug;16(2):2444-2452. doi: 10.3892/ol.2018.8889. Epub 2018 Jun 4.
Lung cancer is one of the major causes of cancer-related deaths worldwide. Notably, miR-155-5p is one of the most amplified miRNAs in non-small cell lung carcinoma (NSCLC). However, the role of miR-155-5p in lung cancer metastasis has not been fully evaluated. In the present study, miR-155-5p mimic and inhibitor were used to investigate the effects of miR-155-5p on the metastasis of human lung carcinoma A549 cells. The study indicated that transfection of miR-155-5p mimic significantly suppressed cell proliferation, migration and invasion of A549 cells, whereas its inhibition significantly promoted cell proliferation, migration and invasion of A549 cells, suggesting a potential therapeutic application of miR-155-5p in controlling lung cancer metastasis. Moreover, transfection of miR-155-5p mimic suppressed the expression of and N-cadherin and induced that of E-cadherin, whereas its inhibition significantly upregulated the expression of Smad2/3, ZEB1, ZEB2 and N-cadherin and downregulated that of E-cadherin. Collectively, the findings suggest that miR-155-5p suppresses the proliferation, migration and invasion of A549 cells. Therefore, loss of miR-155-5p may serve an essential role in tumorigenesis and tumour progression in lung cancers.
肺癌是全球癌症相关死亡的主要原因之一。值得注意的是，miR-155-5p是非小细胞肺癌（NSCLC）中扩增最为明显的miRNA之一。然而，miR-155-5p在肺癌转移中的作用尚未得到充分评估。在本研究中，使用miR-155-5p模拟物和抑制剂来研究miR-155-5p对人肺癌A549细胞转移的影响。研究表明，转染miR-155-5p模拟物可显著抑制A549细胞的增殖、迁移和侵袭，而抑制其表达则显著促进A549细胞的增殖、迁移和侵袭，这表明miR-155-5p在控制肺癌转移方面具有潜在的治疗应用价值。此外，转染miR-155-5p模拟物可抑制Smad2/3和N-钙黏蛋白的表达并诱导E-钙黏蛋白的表达，而抑制其表达则显著上调Smad2/3、ZEB1、ZEB2和N-钙黏蛋白的表达并下调E-钙黏蛋白的表达。总体而言，这些发现表明miR-155-5p可抑制A549细胞的增殖、迁移和侵袭。因此，miR-155-5p的缺失可能在肺癌的肿瘤发生和肿瘤进展中起重要作用。
