Molano Mónica, Buitrago Oscar, Moreno-Acosta Pablo, Garland Suzanne M, Morales Nicolás, Huertas Antonio, Martinez Teresa, Gamboa Oscar, Tabrizi Sepehr N, Cornall Alyssa, Muñoz Nubia
Research Group in Cancer Biology, National Cancer Institute, Bogotá 11001000, Colombia.
Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá 11001000, Colombia.
Oncol Lett. 2018 Aug;16(2):2511-2516. doi: 10.3892/ol.2018.8928. Epub 2018 Jun 8.
Certain variants of human papillomavirus (HPV)type 58 are associated with an increased risk of high grade squamous intraepithelial lesions and cervical cancer. However, little is known about the persistence of HPV58 E6/E7 variants in women with incident HPV58 infections. The aim of the present study was to evaluate the presence and persistence of HPV58 E6/E7 variants in 71 women with incident HPV58 infection throughout their follow-up. These women belonged to a cohort examined in a longitudinal study of 1,610 Colombian women, who were HPV-negative and had normal baseline cytology. E6/E7 DNA regions of HPV58-positive samples were amplified and sequenced using automated direct sequencing. A total of 639 samples were analyzed from the 71 women, and 117 samples (18.3%) were HPV58-positive. HPV58 E6/E7 variants were detected in 85.5% of the samples. The T307/A694/G744/A761 variant was identified in 88% of the samples, the T307/G744 variant was identified in 9% of samples and the T187/T307/A367/G744/G793/T798/A801/T840/C852 was identified in 3% of the samples. Overall, 50% of the HPV58 infections were present after 1 year of follow-up and all infections were cleared after 7 years. Women who had first sexual intercourse at >15 years of age had a lower clearance rate than those who had sexual intercourse for the first time at ≤15 years of age [hazard ratio (HR)=0.29; 95% confidence interval (CI)=0.09-0.92]. Likewise, parous women had a higher clearance rate than nulliparous women (HR=3.43, 95% CI=1.23-9.60). There was no difference in clearance rates between HPV58 E6/E7 variants. In conclusion, HPV58 variants were not associated with persistence of the infection in this group of women.
人乳头瘤病毒58型（HPV58）的某些变体与高级别鳞状上皮内病变和宫颈癌风险增加相关。然而，对于初次感染HPV58的女性中HPV58 E6/E7变体的持续存在情况知之甚少。本研究的目的是评估71例初次感染HPV58的女性在整个随访过程中HPV58 E6/E7变体的存在和持续情况。这些女性属于一项对1610名哥伦比亚女性进行的纵向研究队列，她们HPV检测呈阴性且基线细胞学检查正常。对HPV58阳性样本的E6/E7 DNA区域进行扩增并使用自动直接测序法测序。共对71名女性的639份样本进行了分析，其中117份样本（18.3%）HPV58呈阳性。85.5%的样本中检测到HPV58 E6/E7变体。88%的样本中鉴定出T307/A694/G744/A761变体，9%的样本中鉴定出T307/G744变体，3%的样本中鉴定出T187/T307/A367/G744/G793/T798/A801/T840/C852。总体而言，随访1年后50%的HPV58感染仍然存在，所有感染在7年后清除。首次性交年龄大于15岁的女性清除率低于首次性交年龄小于等于15岁的女性[风险比（HR）=0.29；95%置信区间（CI）=0.09 - 0.92]。同样，经产妇的清除率高于未产妇（HR = 3.43，95% CI = 1.23 - 9.60）。HPV58 E6/E7变体之间的清除率没有差异。总之，在这组女性中，HPV58变体与感染的持续存在无关。
