Department of Neurology, Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang Province 161000, China.
Department of Thoracic Surgery, Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang Province 161000, China.
Gene. 2018 Nov 15;676:117-122. doi: 10.1016/j.gene.2018.07.037. Epub 2018 Jul 17.
Glioma is a devastating disease with increasing incidence worldwide. Circular RNAs (circRNAs) was demonstrated to be involved in a wide range of pathophysiological processes, including tumorigenesis and development. Recently, the abnormally expressed hsa_circ_0001649 was found in several malignancies. In the current study, the expression levels of hsa_circ_0001649 in glioma and its clinical significance were explored. The biological functions including cell growth, colony-forming ability and apoptosis altered by hsa_circ_0001649 were further investigated in vitro. Tumor formation assay was also carried out to elucidate the oncogenic properties of hsa_circ_0001649. The data documented a decrease of hsa_circ_0001649 expression in glioma specimens and cell lines. In addition, down-regulated hsa_circ_0001649 is linked to larger tumor size (p = 0.002) and advanced WHO grade (p = 0.023). Additionally, hsa_circ_0001649 may be an independent prognostic marker for glioma patients after surgery (p = 0.046). Moreover, up-regulated hsa_circ_0001649 inhibits glioma cell growth in vitro and in vivo. Importantly, increased expression of hsa_circ_0001649 facilitates apoptosis by regulating Bcl-2/caspase-3 pathway. Ultimately, this study suggests that hsa_circ_0001649 may be a potential glioma-related prognostic/therapeutic target.
神经胶质瘤是一种具有全球发病率上升趋势的毁灭性疾病。环状 RNA（circRNA）被证明参与广泛的病理生理过程，包括肿瘤发生和发展。最近，几种恶性肿瘤中发现了异常表达的 hsa_circ_0001649。在本研究中，探讨了 hsa_circ_0001649 在神经胶质瘤中的表达水平及其临床意义。进一步研究了 hsa_circ_0001649 改变细胞生长、集落形成能力和凋亡的体外生物学功能。还进行了肿瘤形成实验以阐明 hsa_circ_0001649 的致癌特性。数据记录了神经胶质瘤标本和细胞系中 hsa_circ_0001649 表达的降低。此外，下调的 hsa_circ_0001649 与更大的肿瘤大小（p=0.002）和更高级别的 WHO 分级（p=0.023）相关。此外，hsa_circ_0001649 可能是神经胶质瘤患者手术后的独立预后标志物（p=0.046）。此外，上调的 hsa_circ_0001649 可抑制体外和体内神经胶质瘤细胞的生长。重要的是，通过调节 Bcl-2/caspase-3 通路，增加 hsa_circ_0001649 的表达促进细胞凋亡。最终，本研究表明 hsa_circ_0001649 可能是一种有潜力的神经胶质瘤相关预后/治疗靶标。
