Heit W, Kern P, Heimpel H, Kubanek B
Scand J Haematol. 1977 Feb;18(2):105-12. doi: 10.1111/j.1600-0609.1977.tb02079.x.
The effect of polymorphonuclear granulocytes (PMN) on colony stimulating activity (CSA) was studied in double layer cultures of human Ficoll Isopaque separated white blood cells (mononuclear cells = MNC). Previously published data have been confirmed that granulocytes are able to enhance or inhibit MNC derived CSA. Further analysis of the mode of action of PMN in vitro indicates that the enhancing activity ascribed to granulocytes coincides with low CSA in MNC basal layers. In contrast, in cultures with high levels of CSA as provided by lysed red blood cell enhancement rather than concentrations of PMN are sufficient to induce inhibition of colony growth. A very similar effect to that achieved with basal layer derived CSA could be obtained with conditioned media of PMN and MNC short term liquid cultures. The data indicate, that enhancement and inhibition of colony growth reflect a specific reactivity of granulocytes (PMN) to a given CSA level in the cultures. These findings are discussed in terms of a speculative role of PMN in a negative feed back control mechanism regulating granulopoiesis in vivo.
在人Ficoll Isopaque分离的白细胞（单核细胞=MNC）的双层培养物中研究了多形核粒细胞（PMN）对集落刺激活性（CSA）的影响。先前发表的数据已证实粒细胞能够增强或抑制MNC衍生的CSA。对PMN体外作用方式的进一步分析表明，粒细胞的增强活性与MNC基底层中低水平的CSA一致。相反，在通过裂解红细胞增强提供高水平CSA的培养物中，而不是PMN的浓度足以诱导集落生长的抑制。用PMN和MNC短期液体培养的条件培养基可以获得与基底层衍生的CSA所达到的非常相似的效果。数据表明，集落生长的增强和抑制反映了粒细胞（PMN）对培养物中给定CSA水平的特异性反应性。这些发现根据PMN在体内调节粒细胞生成的负反馈控制机制中的推测作用进行了讨论。
