Ponassi A, Morra L, Bonanni F, Molinari A, Gigli G, Vercelli M, Sacchetti C
Blut. 1979 Oct;39(4):257-63. doi: 10.1007/BF01013218.
Blood colony-forming cells (CFU-C) and colony-stimulating activity obtained from feeder layers of peripheral blood leucocytes (leucocyte CSA) have been studies in 69 normal subjects by means of semisolid agar culture system. Groups of normal volunteers were selected according to sex and age (20 to 45 and older than 60 years) and the results compared. The mean number of circulating CFU-C was significantly lower in young women (20-45 years old) than in males over 60 years of age, but no differences were found among the other age and sex groups. Leucocyte CSA did not significantly differ among these groups. In 5 young males the blood CFU-C did not show significant variations at 8 AM and at 4 PM of the same day. When the study was repeated in 18 subjects at longer time intervals, the number of colonies showed a maximum fivefold variation. The amount of plasma and polymorphonuclear granulocytes present in our culture system did not inhibit the colony growth. In most cases, double layer cultures grow a higher number of colonies than single layer, but feeder layers of some normal subject seem to inhibit the colony growth.
采用半固体琼脂培养系统，对69名正常受试者的血集落形成细胞（CFU-C）和从外周血白细胞饲养层获得的集落刺激活性（白细胞CSA）进行了研究。根据性别和年龄（20至45岁以及60岁以上）选择正常志愿者组并比较结果。年轻女性（20至45岁）循环CFU-C的平均数量显著低于60岁以上男性，但在其他年龄和性别组之间未发现差异。这些组之间白细胞CSA无显著差异。在5名年轻男性中，同一天上午8点和下午4点血CFU-C未显示出显著变化。当对18名受试者进行更长时间间隔的重复研究时，集落数量显示出最大五倍的变化。我们培养系统中存在的血浆和多形核粒细胞数量并未抑制集落生长。在大多数情况下，双层培养比单层培养生长的集落数量更多，但一些正常受试者的饲养层似乎会抑制集落生长。
