Jasim S, Danielsson B R, Tjälve H, Dencker L
Acta Pharmacol Toxicol (Copenh). 1985 Oct;57(4):262-70. doi: 10.1111/j.1600-0773.1985.tb00041.x.
64Cu (as 64CuCl2) was given intravenously to male C57BL mice and to pregnant C57BL mice at various stages of gestation. The disposition of the 64Cu in the adult animals and in the foetuses was studied by autoradiography and gamma spectrometry. The effects of treatments with diethyldithiocarbamate (DEDTC) on the disposition of the 64Cu in the animals were also examined. In addition, the ability of Cu to affect chondrogenesis was studied in an embryonic limb bud culture system. The results showed a strong uptake of 64Cu in the liver of the adult animals at all intervals (5 min.-24 hrs). At short survival intervals, there was also an uptake in the kidney cortex, the gastrointestinal mucosa, the adrenal, the pancreas, and the erythrocytes. Exposure to Cu may cause liver and kidney injuries, which may be related to the strong accumulation in these organs. 64Cu passed the placenta to the foetuses at all stages of gestation, although this occurred at a relatively slow rate. Within the foetuses the highest concentrations were found in the liver. Cu was observed to be toxic in the chick limb bud mesenchymal spot culture system although at relatively high concentrations. Foetal malformations and embryotoxicity may therefore be interpreted as a result of direct action of Cu on embryonic structures, although placental and/or maternal influence cannot be excluded. Pre- or posttreatment of the animals with DEDTC, which is a chelating agent, caused a very marked increase in the concentration of 64Cu in most tissues of the adult animals and also an increased foetal uptake of the metal.(ABSTRACT TRUNCATED AT 250 WORDS)
将64Cu（以64CuCl2形式）静脉注射给雄性C57BL小鼠以及处于不同妊娠阶段的怀孕C57BL小鼠。通过放射自显影和伽马能谱法研究了64Cu在成年动物和胎儿体内的分布情况。还研究了用二乙基二硫代氨基甲酸盐（DEDTC）处理对动物体内64Cu分布的影响。此外，在胚胎肢芽培养系统中研究了铜影响软骨生成的能力。结果显示，在所有时间间隔（5分钟至24小时），成年动物肝脏对64Cu的摄取量都很高。在短存活时间间隔时，肾皮质、胃肠道黏膜、肾上腺、胰腺和红细胞也有摄取。接触铜可能导致肝脏和肾脏损伤，这可能与这些器官中的强烈蓄积有关。在妊娠的各个阶段，64Cu都能通过胎盘进入胎儿体内，不过速度相对较慢。在胎儿体内，肝脏中的浓度最高。在鸡肢芽间充质斑点培养系统中，尽管铜的浓度相对较高，但仍观察到其具有毒性。因此，胎儿畸形和胚胎毒性可能被解释为铜对胚胎结构直接作用的结果，不过不能排除胎盘和/或母体的影响。用螯合剂DEDTC对动物进行预处理或后处理，导致成年动物大多数组织中64Cu的浓度显著增加，同时胎儿对该金属的摄取也增加。（摘要截取自250词）
